Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tom Knight
Date created: 2005-06-06 11:00:00
Date modified: 2015-05-08 01:14:55
pSB1AT3 with ccdB insert
| Types | DnaRegion |
| Roles | plasmid Plasmid |
| Sequences | pSB1AT3_1_sequence (Version 1) |
Description
Target plasmid for three antibiotic assembly. Tetracycline resistant. Contains an insert with a constitutive promoter and the ccdB coding region. The ccdB gene expression is lethal in normal cloning strains. The plasmid must be replicated in the special ccdB resistant strain DB3.1.| Sequence Annotation | Location | Component / Role(s) |
| APr (rev) Tet-r ccdB (rev) ccdA- (rev) BB-Pre BB-Post rep (pMB1) (rev) VF2 VR ORI terminator terminator | 3139,3999 1942,3132 169,474 476,696 120,135 818,836 1076,1690 4042,4061 972,991 1091,1091 90,109 845,879 | CDS feature/cds feature/cds CDS feature/cds CDS feature/cds CDS engineered_region feature/BioBrick engineered_region feature/BioBrick sequence_feature feature/misc feature/primer_binding primer_binding_site primer_binding_site feature/primer_binding sequence_feature feature/misc feature/stem_loop stem_loop stem_loop feature/stem_loop |