pSB1AT3-1

pSB1AT3_1 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:pSB1AT3-1
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tom Knight
Date created: 2005-06-06 11:00:00
Date modified: 2015-05-08 01:14:55

pSB1AT3 with ccdB insert



Types
DnaRegion

Roles
plasmid

Plasmid

Sequences pSB1AT3_1_sequence (Version 1)

Description

Target plasmid for three antibiotic assembly. Tetracycline resistant. Contains an insert with a constitutive promoter and the ccdB coding region. The ccdB gene expression is lethal in normal cloning strains. The plasmid must be replicated in the special ccdB resistant strain DB3.1.

Sequence Annotation Location Component / Role(s)
APr (rev)
Tet-r
ccdB (rev)
ccdA- (rev)
BB-Pre
BB-Post
rep (pMB1) (rev)
VF2
VR
ORI
terminator
terminator
3139,3999
1942,3132
169,474
476,696
120,135
818,836
1076,1690
4042,4061
972,991
1091,1091
90,109
845,879
CDS feature/cds
feature/cds CDS
feature/cds CDS
feature/cds CDS
engineered_region feature/BioBrick
engineered_region feature/BioBrick
sequence_feature feature/misc
feature/primer_binding primer_binding_site
primer_binding_site feature/primer_binding
sequence_feature feature/misc
feature/stem_loop stem_loop
stem_loop feature/stem_loop
igem#experience Works  
igem#sampleStatus Discontinued  
igem#status Deleted  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel pSB1AT3_1/1