Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Austin Che, Leon Chan and Sri Kosuri
Date created: 2006-01-19 12:00:00
Date modified: 2015-05-08 01:14:55
pSB2K3-derived BioBrick plasmid free of many restriction sites
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | pSB2K4_sequence (Version 1) |
Description
Derived from pSB2K3 with many restriction enzyme sites removed by site-directed mutagenesis (~2 dozen mutations). Plasmid is useful when there is a need to cut with a variety of different restriction enzymes. Most symmetric cutters have been removed, and some non-symmetric cutters have also including AarI, AcuI, BbsI, BbvCI, BciVI, BsaI, BsmBI, EarI, N.BstNBI, and SgrAI. Works in exactly same way as pSB2K3. SeeSource
pSB2K3| Sequence Annotation | Location | Component / Role(s) |
| BioBrick suffix E. coli his operon terminator Stem loop T->C C->A Verification reverse f1 ori T->A repE binding site A->G mutation repE binding site incC T->A mutation repE binding site T->C repE binding site repE binding site A->C G->C repE G->C T->C C->T Predicted transcription start Possible -35 Possible -10 Possible -10 Predicted transcription start G->T Possible -10 Possible -35 Predicted transcription start Possible -35 repE binding site repE binding site repE binding site repE binding site oriS Low GC region Low GC region lacOP Maybe A or G T->G T->G repL T->C G->A T->C nnpp promoter 3 (0.95)' Predicted -35 nnpp promoter 2 (0.96)' Predicted -10 Predicted transcription start nnpp promoter 1 (.92)' T->A T->C G->C kanR T->G C->T VF2 (not an exact match, but works) G->C end of terminator Stem loop BioBrick prefix | 1,21 51,120 59,91 93,93 146,146 184,203 269,317 292,292 394,412 462,462 477,495 394,641 551,551 568,586 578,578 597,619 623,641 692,692 857,857 644,1399 1091,1091 1100,1100 1142,1142 1426,1426 1426,1431 1433,1441 1444,1452 1447,1447 1454,1454 1455,1463 1455,1460 1460,1460 1477,1482 1497,1515 1527,1537 1552,1570 1581,1591 1490,1706 1636,1682 1823,1844 2064,2238 2565,2565 2567,2567 2699,2699 2316,3161 2774,2774 3017,3017 3251,3251 3269,3314 3359,3364 3353,3398 3379,3387 3394,3394 3375,3420 3507,3507 3702,3702 3849,3849 3460,4275 3903,3903 4119,4119 4313,4332 4321,4321 4389,4392 4402,4421 4446,4467 | sequence_feature feature/dna stem_loop feature/stem_loop stem_loop feature/stem_loop sequence_alteration feature/mutation feature/mutation sequence_alteration primer_binding_site feature/primer_binding sequence_feature feature/dna sequence_alteration feature/mutation feature/operator operator sequence_alteration feature/mutation feature/operator operator feature/misc sequence_feature sequence_alteration feature/mutation feature/operator operator sequence_alteration feature/mutation feature/operator operator feature/operator operator feature/mutation sequence_alteration feature/mutation sequence_alteration CDS feature/cds sequence_alteration feature/mutation sequence_alteration feature/mutation sequence_alteration feature/mutation feature/misc sequence_feature feature/promoter promoter promoter feature/promoter promoter feature/promoter feature/misc sequence_feature sequence_alteration feature/mutation feature/promoter promoter feature/promoter promoter feature/misc sequence_feature feature/promoter promoter feature/operator operator feature/operator operator feature/operator operator feature/operator operator sequence_feature feature/dna sequence_feature feature/misc sequence_feature feature/misc promoter feature/promoter feature/misc sequence_feature sequence_alteration feature/mutation feature/mutation sequence_alteration CDS feature/cds sequence_alteration feature/mutation sequence_alteration feature/mutation feature/mutation sequence_alteration feature/promoter promoter promoter feature/promoter feature/promoter promoter promoter feature/promoter sequence_feature feature/misc promoter feature/promoter point_mutation feature/s_mutation point_mutation feature/s_mutation point_mutation feature/s_mutation CDS feature/cds point_mutation feature/s_mutation point_mutation feature/s_mutation primer_binding_site feature/primer_binding feature/mutation sequence_alteration feature/stem_loop stem_loop feature/stem_loop stem_loop feature/dna sequence_feature |