Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Reshma Shetty
Date created: 2004-06-28 11:00:00
Date modified: 2015-05-08 01:14:55
pSB4A3 (Replaced by pSB4A5)
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | pSB4A3_sequence (Version 1) |
Description
pSB4A3-1 is a low copy plasmid with ampicillin resistance. It has a repA pSC101-derived replication origin. (Copy number ~10-12, Lutz and Bujard, 1997.)pSB4A3 has a terminator upstream of its MCS, which is oriented to prevent transcription from *inside* the MCS from reading out into any DNA outside the MCS.The orientation of the pBLA promoter driving the AmpR gene is such that relatively little transcription should be coming into the MCS from upstream. A second terminator (E.coli His operon-derived) is downstream of the MCS, again insulating the vector from transcription reading out of the MCS. Ideally, future versions of standard biobrick vectors would have terminators bracketing the MCS that were 100% efficient in terminating transcription both into and out of the MCS region.
| Sequence Annotation | Location | Component / Role(s) |
| rbs(rev) -10 -35 end of terminator prefix suffix E.coli His operon terminator repA (rev) RBS (rev) (rev) AmpR (rev) Terminator (rev) VR VF2 deletion of two bases terminator stem loop terminator stem loop | 3085,3089 3098,3103 3124,3129 3276,3279 3318,3339 1,21 22,93 384,1334 1338,1350 1355,1374 2220,3080 2145,2174 157,176 3200,3219 34,35 30,64 3289,3308 | ribosome_entry_site feature/rbs feature/promoter promoter feature/promoter promoter feature/misc sequence_feature feature/BioBrick engineered_region feature/BioBrick engineered_region sequence_feature feature/misc feature/cds CDS ribosome_entry_site feature/rbs feature/promoter promoter CDS feature/cds feature/stem_loop stem_loop feature/primer_binding primer_binding_site primer_binding_site feature/primer_binding feature/misc sequence_feature stem_loop feature/stem_loop stem_loop feature/stem_loop |