BBa_K648031
BBa_K648031 Version 1 (Component)
RBS with cI repressor

BBa_K1104902
BBa_K1104902 Version 1 (Component)
pTet + RBS + cI + Term

BBa_K1773008
BBa_K1773008 Version 1 (Component)
pLux/cI Right promoter with strong RBS

BBa_I9005
BBa_I9005 Version 1 (Component)
nor_input (tp901-cI) B0034.C0075

BBa_K091230
BBa_K091230 Version 1 (Component)
cI promoter+RBS+RFP+TT

BBa_I744111
BBa_I744111 Version 1 (Component)
Tc-driven cI (lambda) generator

BBa_I10200
BBa_I10200 Version 1 (Component)
434 cI repressor protein generator

BBa_K1647000
BBa_K1647000 Version 1 (Component)
CI operator site with GFP

BBa_J13101
BBa_J13101 Version 1 (Component)
LacI repressed lambda CI generator

PL GFP
BBa_K193000 Version 1 (Component)
GFP reporter regulated by CI.

BBa_K1053300
BBa_K1053300 Version 1 (Component)
PLtetO-1-RBS-cI-DT

BBa_K1421002
BBa_K1421002 Version 1 (Component)
plux R+RBS+cI+2TE

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K909004
BBa_K909004 Version 1 (Component)
cI with strong ribosomal binding site

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_I719022
BBa_I719022 Version 1 (Component)
cI promoter with GFP reporter

BBa_K648032
BBa_K648032 Version 1 (Component)
Slightly weaker RBS with cI repressor

BBa_K648033
BBa_K648033 Version 1 (Component)
Very weak RBS with cI repressor

BBa_K318509
BBa_K318509 Version 1 (Component)
lacI pL + RBS + cI LVA + TT

Plamda-GFP
BBa_I763011 Version 1 (Component)
promoter lambda (cI regulated) with GFP (+LVA) reporter

BBa_K1707004
BBa_K1707004 Version 1 (Component)
TetR-ssRA repressor with the promoter of cI

BBa_K188222
BBa_K188222 Version 1 (Component)
RBS+cI repressor from phage 434 (+LVA)

BBa_K415011
BBa_K415011 Version 1 (Component)
PtetR : RBS : LuxR : Term : PluxR/cI-OR : RBS : mCherry : Term : Plux/cI-OR : RBS : LuxI

BBa_K1412088
BBa_K1412088 Version 1 (Component)
A combination of theophylline aptamer and taRNA that can response theophylline to regulate circuit

BBa_K077039
BBa_K077039 Version 1 (Component)
cI under control of the plac promotor

BBa_K176017
BBa_K176017 Version 1 (Component)
pCI(R0051)(lambda CI-)->RBS+GFP+T

BBa_J06570
BBa_J06570 Version 1 (Component)
Construction intermediate: 434 cI with RBS (B0034.C0052)

BBa_K145105
BBa_K145105 Version 1 (Component)
cI under T7 and P_R dual promotor

BBa_I3401
BBa_I3401 Version 1 (Component)
Lambda cI switch input device (B0034.C0051.B0015)

BBa_K584011
BBa_K584011 Version 1 (Component)
Lac-Lux hybrid promotor + CrtEBI + CI repressor + INP

BBa_K611017
BBa_K611017 Version 1 (Component)
cI Lambda Repressor and Promoter Wild Type Control

PLac-LacY-
BBa_I763013 Version 1 (Component)
LacY and cI coding device switched on by IPTG

BBa_I758601
BBa_I758601 Version 1 (Component)
Screen for binding affinity of mutant cI lambda to promotor sites

BBa_I758600
BBa_I758600 Version 1 (Component)
Screen for binding affinity of mutant cI lambda to promotor sites

BBa_K145112
BBa_K145112 Version 1 (Component)
cI under T7 and PR_R dual promotor

BBa_K177035
BBa_K177035 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA) under control of RBS.3 (medium)

BBa_K584008
BBa_K584008 Version 1 (Component)
Lambda cI and LuxR regulated hybrid promotor + RBS + MelA + RBS + AFP + term

BBa_K415005
BBa_K415005 Version 1 (Component)
pLux/cI-OR : RBS-mCherry : Term : p(tetR) : RBS-luxR : Term

iGEM Parts Registry
igem_collection Version 1 (Collection)
The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.