Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

ACS Synbio Generation
ACS_Synbio_Generation Version 1 (Activity)
Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

Sequence Cut Out of Plasmid
cut_out_of_plasmid Version 1 (Activity)
Used to indicate that a sequence was cut out of the plasmid referenced as the source

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

Composite Sequence Pull
Composite Version 1 (Activity)
When multiple sources where combined to make a new part.

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

Amp Cas
BBa_I13800 Version 1 (Component)
Ampicillin Resistance Cassette

BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

BBa_K1075041
BBa_K1075041 Version 1 (Component)
RBS32-MazE-(Ec)ssrA(DAS+4)

BBa_J52008
BBa_J52008 Version 1 (Component)
luciferase: luciferin 2-monooxygenase from Renilla reniformis (EC 1.13.12.5; SwissProt:: P27652)

BBa_K1075043
BBa_K1075043 Version 1 (Component)
pLac-RBS32-mazE-(Ec)ssrA(DAS+4)-TT

BBa_K1321005
BBa_K1321005 Version 1 (Component)
Synthetic Phytochelatin EC(20) RFC[25]

BBa_K1075042
BBa_K1075042 Version 1 (Component)
RBS32-MazE-(Ec)ssrA(DAS+4)-TT

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

BBa_K1075024
BBa_K1075024 Version 1 (Component)
RBS34- mCherry- (Ec)ssrA(DAS+4)-TT

BBa_K1075048
BBa_K1075048 Version 1 (Component)
AraC-pBAD-RBS32-mazF-TT-pLac-RBS32-mazE-(Ec)ssrA(DAS+4)-TT

BBa_K1075025
BBa_K1075025 Version 1 (Component)
pLac-RBS34-mCherry-(Ec)ssrA(DAS+4)-TT

ssrA(DAS+4
BBa_K1075020 Version 1 (Component)
E. coli ssrA(DAS+4)

BBa_K1075023
BBa_K1075023 Version 1 (Component)
mCherry- (Ec)ssrA(DAS+4)-TT

BBa_K1075026
BBa_K1075026 Version 1 (Component)
AraC-pBad-RBS34-mCherry-(Ec)ssrA(DAS+4)-TT

BBa_K1075022
BBa_K1075022 Version 1 (Component)
mCherry- (Ec)ssrA(DAS+4)

BBa_K1789002
BBa_K1789002 Version 1 (Component)
RBS30

BBa_K1075027
BBa_K1075027 Version 1 (Component)
SplitSspB(Rapamycin inducable)-pLac-RBS34-mCherry-(Ec)ssrA(DAS+4)-TT

BBa_K1075038
BBa_K1075038 Version 1 (Component)
RBS32-MazF-TT

Las CIO
BBa_I14016 Version 1 (Component)
P(Las) CIO

BBa_K1399007
BBa_K1399007 Version 1 (Component)
GFP (mut3b) with SsrA-DAS+2 degradation tag

BBa_K2120008
BBa_K2120008 Version 1 (Component)
mazF

BBa_K1075033
BBa_K1075033 Version 1 (Component)
RBS32-ccdA-ssrA

BBa_K1399008
BBa_K1399008 Version 1 (Component)
GFP (mut3b) with DAS-ssrA degradation tag

BBa_K1075021
BBa_K1075021 Version 1 (Component)
E. coli ssrA(DAS+4)-TT

BBa_K302033
BBa_K302033 Version 1 (Component)
mazF

BBa_K302032
BBa_K302032 Version 1 (Component)
mazE

dxs
BBa_K118000 Version 1 (Component)
dxs coding sequence encoding 1-deoxyxylulose-5-phosphate synthase

BBa_K1075034
BBa_K1075034 Version 1 (Component)
RBS32-ccdA-ssrA-TT

BBa_K1075040
BBa_K1075040 Version 1 (Component)
AraC-pBAD-RBS32-mazF-TT

BBa_K1075039
BBa_K1075039 Version 1 (Component)
pLac-RBS32-mazF-TT

BBa_K1075046
BBa_K1075046 Version 1 (Component)
pDawn-RBS32-mazF-TT

BBa_K1399003
BBa_K1399003 Version 1 (Component)
RFP from Discosoma striata (coral) with DAS-ssrA degradation tag

BBa_K1075035
BBa_K1075035 Version 1 (Component)
pLac-RBS32-ccdA-ssrA-TT

BBa_K1075036
BBa_K1075036 Version 1 (Component)
AraC-pBAD-RBS32-ccdA-ssrA-TT

dCas 9
BBa_K1051800 Version 1 (Component)
protein dCas9.Two mutations,D10A and H841A,from Cas9 gene without stop codon.

BBa_K1399005
BBa_K1399005 Version 1 (Component)
GFP (mut3b) with AAV-ssrA degradation tag

BBa_M0052
BBa_M0052 Version 1 (Component)
AANDENYADAS (moderately fast) SsrA degradation tag.

BBa_K1399004
BBa_K1399004 Version 1 (Component)
GFP (mut3b) with LVA-ssrA degradation tag