BBa_K346093BBa_K346093 Version 1 (Component)Constutitive promoter BBa_J23103+RBS(BBa_B0034)+PpbrR
BBa_K346009BBa_K346009 Version 1 (Component)Constutitive promoter(BBa_J23103)+RBS+MerR(BBa_K346001)
BBa_K1968010BBa_K1968010 Version 1 (Component)PgdaA constituve promoter from Aspergillus niger
BBa_K290001BBa_K290001 Version 1 (Component)constitutive RhlR with bicistronic LuxI - GFP controlled by pRhl
BBa_K332034BBa_K332034 Version 1 (Component)Constitutive promoter + 37℃ induced RBS + tetR + double terminator
BBa_K346078BBa_K346078 Version 1 (Component)Constutitive promoter BBa_J23109+pag activator(BBa_I746352)
BBa_K1437006BBa_K1437006 Version 1 (Component)mouse AhR detective protein 83-805 with terminator codon optimized for yeast
BBa_K1154006BBa_K1154006 Version 1 (Component)Mating pheromone-induced IGPD and constitutive LDH expression in yeast
BBa_K079032BBa_K079032 Version 1 (Component)GFP reporter protein under the control of the BBa_J23100 constitutive promoter
BBa_S03737BBa_S03737 Version 1 (Component)pLac-lox-RFP(reverse)-TT-lox-RBS-Tet (psB1A2)
PrtDEFBBa_K258007 Version 1 (Component)Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi
Plac/ara-1BBa_K1145006 Version 1 (Component)Drive the expression of LuxRI in pLuxRI2 and its derivatives.
BBa_K1088058BBa_K1088058 Version 1 (Component)10 aa linker with BamHI restriction site and TEV recognition site
BBa_K1638006BBa_K1638006 Version 1 (Component)10 aa linker with BamHI restriction site and TEV recognition site
BBa_K1968013BBa_K1968013 Version 1 (Component)PgdaA constituve Phytobrick promoter: Glyceraldehyde-3-phosphate dehydrogenase from Aspergillus nige
BBa_K137033BBa_K137033 Version 1 (Component)Device with GFP with (AC)21 repeat after start codon
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.