BBa_K1351019BBa_K1351019 Version 1 (Component)Reverse complementary RNA sequence which binds the mRNA of the SdpI immunity
ssTorA_CS-BBa_K627012 Version 1 (Component)Fusion of TorA sig-seq, TEV protease cleavage site and b-lactamase
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
BBa_K1113411BBa_K1113411 Version 1 (Component)Targeting sequence for the delivery of the LacZ gene to the Carboxysome
BBa_K1412088BBa_K1412088 Version 1 (Component)A combination of theophylline aptamer and taRNA that can response theophylline to regulate circuit
BBa_K541715BBa_K541715 Version 1 (Component)Multi-host vector pTG262 converted to BioBrick vector wtih LALF protein and SacB signal peptide
BBa_K2150010BBa_K2150010 Version 1 (Component)This part consists of a gene encoding toxin 134 with a LacI gene, a Ptac promoter included