BBa_K2150010

BBa_K2150010 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K2150010
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zepeng Mu
Date created: 2016-10-05 11:00:00
Date modified: 2016-10-13 10:02:17

This part consists of a gene encoding toxin 134 with a LacI gene, a Ptac promoter included



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K2150010_sequence (Version 1)

Description

This part contain sequence encoding EndoA, a toxin gene in bacteria.

Notes

We used RBS BBa_B0033 to reduce leakage, and there is a RiboJ (an insulator element) directly upstream to the CDS in order to reduce the background noise.

Source

This gene is acquired by PCR from genome of Bacillus subtilis

Access Instance Definition
public
BBa_B0033
BBa_B0033
Sequence Annotation Location Component / Role(s)
BBa_B0033
1,11
BBa_B0033
igem#experience
None
 
igem#status
Deleted
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2150010/1