BBa_K1085020
BBa_K1085020 Version 1 (Component)
RBS Start-codon EstA SpiderSilkSubunitN1 SpiderSilkSubunitN2 SilkTail Strep-tag Stop-codon

BBa_K1085019
BBa_K1085019 Version 1 (Component)
RBS Start-codon EstA Strep-tag SpiderSilkSubunitE1 SpiderSilkSubunitE2 SilkTail Stop-codon

BBa_K1085028
BBa_K1085028 Version 1 (Component)
RBS Start-codon SpiderSilkSubunitE1 SpiderSilkSubunitE2 x2 SilkTail Strep-tag Stop-codon

BBa_K235036
BBa_K235036 Version 1 (Component)
[K235030][K235005] (constituitive promoter+32??C RBS thermometer+mCherry CDS+stop)

BBa_J119395
BBa_J119395 Version 1 (Component)
tClone Red containing the RS3 Riboswitch

BBa_K1848003
BBa_K1848003 Version 1 (Component)
Human Gut Hormone Ghrelin (28) (Sequence Lacks Stop Codon, but part possesses it)

BBa_K112212
BBa_K112212 Version 1 (Component)
{rbs.int!} The integrase gene with native rbs site and stop codon, BBb format

BBa_K323080
BBa_K323080 Version 1 (Component)
CMV ATG cYFP link Zif268 link His stop - part of mammalian split/FRET system

BBa_K323029
BBa_K323029 Version 1 (Component)
CMV ATG cCFP link ZNF HIVC His stop - part of mammalian split/FRET system

BBa_K1162014
BBa_K1162014 Version 1 (Component)
protmoter+rbs+N-terminal 10x His-Tag+GFP with no translational stop codon

xis
BBa_K112205 Version 1 (Component)
{rbs.xis!} The xis gene with native rbs site and stop codon; assembly standard 21

BBa_K1051036
BBa_K1051036 Version 1 (Component)
BBa_K1051105(non stop codon to Mitochondria Outer Membrane)+BBa_K1051008( CAA terminator with stopco

BBa_K892999
BBa_K892999 Version 1 (Component)
red light responsive inverter driving luciferase

BBa_K1848002
BBa_K1848002 Version 1 (Component)
Human Gut Hormone Peptide YY (3-36) (Sequence Lacks Stop Codon, but part possesses it)

BBa_K1456012
BBa_K1456012 Version 1 (Component)
Human Tissue Plasminogen Activator reverse primer with restriction site, 2 stop codons and 6X His

BBa_K1456010
BBa_K1456010 Version 1 (Component)
Glutathione Peroxidase (GPx) enzyme reverse primer with restriction site, two stop codon and 6X His

BBa_K1456008
BBa_K1456008 Version 1 (Component)
Superoxide Dismutase enzyme reverse primer with restriction site, two stop codon and 6X His-Tag

halt
BBa_M36296 Version 1 (Component)
Stop Codon TAA

BBa_K1456022
BBa_K1456022 Version 1 (Component)
Glutathione Peroxidase(GPx1) enzyme reverse primer-2 with restriction site, two stop codon and 6xHis

BBa_K1456024
BBa_K1456024 Version 1 (Component)
Human Tissue Plasminogen Activator reverse primer-2 with restriction site, 2 stop codons and 6XHis

BBa_K112217
BBa_K112217 Version 1 (Component)
{a~ihfA!} The ihf alpha gene ready to have rbs attached with stop codon, BBb format

BBa_K112223
BBa_K112223 Version 1 (Component)
{a~ihfB!} The ihf beta gene ready to have rbs attached and stop codon, BBb format

BBa_K112211
BBa_K112211 Version 1 (Component)
{a~int!} The integrase gene with rbs ready to be attached with stop codon, BBb format

BBa_K1332003
BBa_K1332003 Version 1 (Component)
The 5?? side of the intron(+exon fragment) from td gene of T4 phage without stop codon

xis
BBa_K112204 Version 1 (Component)
{a~xis!} The bacteriophage lambda xis gene ready to have rbs attached and stop codon; assembly stand

BBa_K1848001
BBa_K1848001 Version 1 (Component)
Human Gut Hormone Glucagon-like peptide 1 (7-37) (Sequence Lacks Stop Codon, but part possesses it)

BBa_K209000
BBa_K209000 Version 1 (Component)
cAR1 (Dictyostelium discoideum), no STOP

BBa_K1051356
BBa_K1051356 Version 1 (Component)
K1051301(clb2 promoter) + K1051053(K1051001 (non stop codon ECFP + K1051006 (Stop codon + TBY-1 term

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.