BBa_K1412001
BBa_K1412001 Version 1 (Component)
Endow the E.coli engineered strain(CL-1) the ability of chemotaxis

BBa_K648027
BBa_K648027 Version 1 (Component)
c1 repressor with a very weak RBS on a promoter

BBa_K238028
BBa_K238028 Version 1 (Component)
ech with RBS

BBa_K1075027
BBa_K1075027 Version 1 (Component)
SplitSspB(Rapamycin inducable)-pLac-RBS34-mCherry-(Ec)ssrA(DAS+4)-TT

BBa_I742011
BBa_I742011 Version 1 (Component)
ech (feruloyl hydratase/aldolase; vanillin biosynthesis)

BBa_I719001
BBa_I719001 Version 1 (Component)
ech and fcs genes

BBa_I719011
BBa_I719011 Version 1 (Component)
ech and fcs genes

BBa_K1444004
BBa_K1444004 Version 1 (Component)
Composite promoter and weak <i>B. subtilis </i> RBS - <i>C1-&#955;</i>

BBa_K1075048
BBa_K1075048 Version 1 (Component)
AraC-pBAD-RBS32-mazF-TT-pLac-RBS32-mazE-(Ec)ssrA(DAS+4)-TT

BBa_K1412600
BBa_K1412600 Version 1 (Component)
Endow the CL-1(E.coli engineered strain) the ability of chemotaxis and quorum sensing

BBa_I754000
BBa_I754000 Version 1 (Component)
Feruloyl CoA Hyratase for vanillin biosynthesis-ech

BBa_K1124108
BBa_K1124108 Version 1 (Component)
pLambda-mCherry-pConst-c1 repressor generator (-LVA, -barcode, E234K) (for UV induction check)

BBa_K1444003
BBa_K1444003 Version 1 (Component)
Composite promoter and consensus <i>B. subtilis</i> RBS - <i>C1-&#955;</i> x3

BBa_I726005
BBa_I726005 Version 1 (Component)
Trc-LC

BBa_I754001
BBa_I754001 Version 1 (Component)
ech protein generator for vanillin biosynthesis

BBa_I769106
BBa_I769106 Version 1 (Component)
ech protein generator for vanillin biosynthesis

BBa_I735000
BBa_I735000 Version 1 (Component)
ech gene coding sequence for feruoyl CoA hydratese

BBa_K195633
BBa_K195633 Version 1 (Component)
RBS + cl repressor + terminator

BBa_I718001
BBa_I718001 Version 1 (Component)
ech My new test generator part Feruloyl CoA hydratase for vanilin

BBa_K1444010
BBa_K1444010 Version 1 (Component)
Composite promoter and weak B. subtilis RBS - C1-434

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.