Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dan Ziemianowicz
Date created: 2014-10-07 11:00:00
Date modified: 2015-05-08 01:10:27
Composite promoter and weak B. subtilis RBS - C1-λ
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K1444004_sequence (Version 1) |
Description
A composite part consisting of the constitutive promoter Pveg (BBa_K823003), followed by the repressible promoter C1-lambda (BBa_R0051), and ending with and ending with the weak RBS from B. subtilis (BBa_K780002).This composite part was intended to control the expression of a downstream reporter, regulated by the expression of the pLacI repressor protein. The constitutive promoter Pveg allows reliable expression when the repressible promoter is not repressed.
Notes
No interference with endogenous cell signalling.Source
B. subtilis| Access | Instance | Definition |
| public public public | BBa_K823003 BBa_R0051 BBa_K780002 | BBa_K823003 cI lam R37 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K823003 BBa_R0051 BBa_K780002 | 1,237 246,294 303,318 | BBa_K823003 cI lam R37 |