Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tangduo Zhang
Date created: 2014-08-27 11:00:00
Date modified: 2015-05-08 01:10:18
Endow the E.coli engineered strain(CL-1) the ability of chemotaxis
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K1412001_sequence (Version 1) |
Description
What it is: Composite part enables the chemotaxis of our engineering bacteria could be regulated by IPTG and L-Arabinose; pBAD and pLac promoters control the expression of Lac I and CheZ.What it does: when L-Arabinose is added, pBAD promoter is activated, expressing Lac I, inhibiting the expression of CheZ. When IPTG is added, IPTG combines with Lac I, forming a complex, thus relief the inhibition and the CheZ express, make the ability of chemotaxis recover.
How to use it in their project: Use IPTG as an inducer and L-Arabinose as an inhibitor to take control of the chemotaxis of the engineering bacteria.
Notes
chemotaxisSource
14A 2014 Kit Plate 3 pSB1C36F 2014 Kit Plate 2 pSB1C3
2M 2013 Kit Plate 5 pSB1A2
4F 2013 Kit Plate 3 pSB1C3
1N 2014 Kit Plate 3 pSB1C3
2N 2014 Kit Plate 2 pSB1C3
18G 2014 Kit Plate 1 pSB1C3
4G 2014 Kit Plate 3 pSB1C3
3H 2013 Kit Plate 3 pSB1C3
| Access | Instance | Definition |
| public public public public public public | BBa_K206000 BBa_I732820 BBa_R0010 BBa_B0034 BBa_K629003 BBa_B0015 | pBAD BBa_I732820 LacI BBa_B0034 cheZ BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K206000 BBa_I732820 BBa_R0010 BBa_B0034 BBa_K629003 BBa_B0015 | 1,130 139,1379 1388,1587 1596,1607 1614,2258 2267,2395 | pBAD BBa_I732820 LacI BBa_B0034 cheZ BBa_B0015 |