BBa_K917001
BBa_K917001 Version 1 (Component)
ars promoter plus cscA sucrose assimilation gene

BBa_K823051
BBa_K823051 Version 1 (Component)
mKate2, a red monomeric fluorescent protein + RBS + Term

BBa_K2170001
BBa_K2170001 Version 1 (Component)
Secretory eukaryotic biotin binding receptor with enhanced monomeric avidin

BBa_K2170050
BBa_K2170050 Version 1 (Component)
Secretory prokaryotic biotin binding receptor with enhanced monomeric avidin

BBa_K1739002
BBa_K1739002 Version 1 (Component)
Sequence coding for Sup35 with a N-terminal <i>CsgA</i> signal sequence for amyloid export

BBa_K1583016
BBa_K1583016 Version 1 (Component)
CsgB

BBa_K1124106
BBa_K1124106 Version 1 (Component)
plambda-sRNA(anti-tyrR)-plambda-sRNA (anti-csrA) (tyrosine synthesis device)

BBa_K805015
BBa_K805015 Version 1 (Component)
CsgD, the master regulator of biofilm formation

BBa_K1638037
BBa_K1638037 Version 1 (Component)
CsrA fused to T25 domain of CyaA with cAMP-induced RFP reporter

BBa_I10101
BBa_I10101 Version 1 (Component)
csgD

BBa_K1124107
BBa_K1124107 Version 1 (Component)
pLac-hpaBC-plambda-sRNA(anti-tyrR)-plambda-sRNA (anti-csrA) (L-DOPA synthesis device)

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.