BBa_K199155
BBa_K199155 Version 1 (Component)
Measures the reverse activity of pLux in presence of pOmpC

BBa_K497019
BBa_K497019 Version 1 (Component)
Reverse primer Deinococcus radiodurans bacteriophytochrome gene for insertion after heme oxygenase

BBa_K1351019
BBa_K1351019 Version 1 (Component)
Reverse complementary RNA sequence which binds the mRNA of the SdpI immunity

BBa_J70343
BBa_J70343 Version 1 (Component)
J70315psri.r.1: J70315 psri reverse part for pcr of S03621 (single stranded)

BBa_K199153
BBa_K199153 Version 1 (Component)
Measures the reverse activity of pLux in absence of LuxR

BBa_K199154
BBa_K199154 Version 1 (Component)
Measures the reverse activity of pLux in absence of LuxR

BBa_K199152
BBa_K199152 Version 1 (Component)
Measures the reverse activity of pLux in the presence of LuxR

BBa_K497017
BBa_K497017 Version 1 (Component)
Reverse primer for Agrobacterium tumefaciens bacteriophytochrome gene for insertion after heme oxyge

BBa_K093011
BBa_K093011 Version 1 (Component)
reverse B0015

BBa_K1456014
BBa_K1456014 Version 1 (Component)
Oxygen dependent degradation(ODD) domain from HIF-1a reverse primer with restriction site

BBa_I51001
BBa_I51001 Version 1 (Component)
BioBrick base vector, as revised by Codon Devices

BBa_J102001
BBa_J102001 Version 1 (Component)
Reverse Lux Promoter

M13 -47
BBa_K176059 Version 1 (Component)
M13 -47 general primer as a reverse primer binds to 5prime terminal of lacZ

BBa_K1456020
BBa_K1456020 Version 1 (Component)
Superoxide dismutase-1 (SOD-1) reverse primer-2 with restriction site and kozak sequence

loxBri (R)
BBa_K315006 Version 1 (Component)
LoxBri (R)variant reverse lox site with 3 base changes in the spacer region

BBa_K1456012
BBa_K1456012 Version 1 (Component)
Human Tissue Plasminogen Activator reverse primer with restriction site, 2 stop codons and 6X His

BBa_K1456010
BBa_K1456010 Version 1 (Component)
Glutathione Peroxidase (GPx) enzyme reverse primer with restriction site, two stop codon and 6X His

BBa_K1456008
BBa_K1456008 Version 1 (Component)
Superoxide Dismutase enzyme reverse primer with restriction site, two stop codon and 6X His-Tag

BBa_K1456022
BBa_K1456022 Version 1 (Component)
Glutathione Peroxidase(GPx1) enzyme reverse primer-2 with restriction site, two stop codon and 6xHis

BBa_K1456024
BBa_K1456024 Version 1 (Component)
Human Tissue Plasminogen Activator reverse primer-2 with restriction site, 2 stop codons and 6XHis

BBa_J3109
BBa_J3109 Version 1 (Component)
RFP Reverse

BBa_M36689
BBa_M36689 Version 1 (Component)
HSE 2x Promoter + Bxb1 Recombinase w/ NLS + SV40 Terminator + attB site + CMV Reverse + attP site

BBa_I742129
BBa_I742129 Version 1 (Component)
Reverse terminator BBa_B0011 LuxICDABEG (+/-)

BBa_K206008
BBa_K206008 Version 1 (Component)
pBAD reverse - terminator

BBa_K187057
BBa_K187057 Version 1 (Component)
ispU ORF reverse primer

BBa_K187323
BBa_K187323 Version 1 (Component)
kdsB ORF, Reverse Primer

C2/RGE
BBa_K1442105 Version 1 (Component)
C2 Testing Module (reverse GFP)

BBa_I741013
BBa_I741013 Version 1 (Component)
Reverse Total XylR Transciptional Regulator

BBa_J70459
BBa_J70459 Version 1 (Component)
yfp RBS, {0,5;15,10} family member - B0031 simulator (reverse oligo)

BBa_S03737
BBa_S03737 Version 1 (Component)
pLac-lox-RFP(reverse)-TT-lox-RBS-Tet (psB1A2)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.