BBa_K581003BBa_K581003 Version 1 (Component)SgrS2+Terminator (small RNA regulator, conjugate part of ptsG2)
BBa_M31116BBa_M31116 Version 1 (Component)M13 Gene VIII terminator and promoter of Gene X
BBa_K077041BBa_K077041 Version 1 (Component)AiiA and cII under control of plac promotor
KanR-BA-BfBBa_K349012 Version 1 (Component)KanR-BA-BfuAI (for construction of BA BioBytes 2.0)
BBa_K648123BBa_K648123 Version 1 (Component)RecA with mutation of Lys 286 and Arg 243
BBa_K1088057BBa_K1088057 Version 1 (Component)T25 domain of bacterial two-hybrid system (IPTG inducible)
BBa_K1172914BBa_K1172914 Version 1 (Component)Part 2 of the Biosafety-System TetOR alive (TetO GFP)
AraC_TEV-FBBa_K627008 Version 1 (Component)Fusion part of arabinose-inducible induction system and the TEV protease
BBa_I758601BBa_I758601 Version 1 (Component)Screen for binding affinity of mutant cI lambda to promotor sites
BBa_I758600BBa_I758600 Version 1 (Component)Screen for binding affinity of mutant cI lambda to promotor sites
BBa_K1351019BBa_K1351019 Version 1 (Component)Reverse complementary RNA sequence which binds the mRNA of the SdpI immunity
ssTorA_CS-BBa_K627012 Version 1 (Component)Fusion of TorA sig-seq, TEV protease cleavage site and b-lactamase
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
BBa_K1113411BBa_K1113411 Version 1 (Component)Targeting sequence for the delivery of the LacZ gene to the Carboxysome
BBa_K1412088BBa_K1412088 Version 1 (Component)A combination of theophylline aptamer and taRNA that can response theophylline to regulate circuit
BBa_K2150010BBa_K2150010 Version 1 (Component)This part consists of a gene encoding toxin 134 with a LacI gene, a Ptac promoter included