BBa_I759035
BBa_I759035 Version 1 (Component)
cis2-repressed, tet-regulated YFP

BBa_K528008
BBa_K528008 Version 1 (Component)
RBS measurement device J23100 B0032 YFP

BBa_K189060
BBa_K189060 Version 1 (Component)
Gp15 of Bacteriophage Mu

BBa_K086000
BBa_K086000 Version 1 (Component)
unmodified Lutz-Bujard LacO promoter - YFP

BBa_K1616003
BBa_K1616003 Version 1 (Component)
VVD link to YC155 (YFP Cter split)

BBa_K1616004
BBa_K1616004 Version 1 (Component)
VVD linked to YN155 (YFP Nter split)

BBa_I12026
BBa_I12026 Version 1 (Component)
Test of BBa_R0011 (LacI regulated) using YFP

BBa_I15024
BBa_I15024 Version 1 (Component)
Tet-repressible polycistronic CFP/YFP under B0034

BBa_J70459
BBa_J70459 Version 1 (Component)
yfp RBS, {0,5;15,10} family member - B0031 simulator (reverse oligo)

BBa_K1014015
BBa_K1014015 Version 1 (Component)
Biological proportional operational Mu-circuit(1)

BBa_J24823
BBa_J24823 Version 1 (Component)
same as J24819 but with ACCACC Euk RBS removed and problem solved via J24822 removed

BBa_I13273
BBa_I13273 Version 1 (Component)
YFP Producer Controlled by 3OC₆HSL Receiver Device

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.