BBa_J48104BBa_J48104 Version 1 (Component)NikR promoter, a protein of the ribbon helix-helix family of trancription factors that repress expre
BBa_M36305BBa_M36305 Version 1 (Component)Transcription terminator pT-T7 from bacteriophage
BBa_K648032BBa_K648032 Version 1 (Component)Slightly weaker RBS with cI repressor
BBa_K1114500BBa_K1114500 Version 1 (Component)MoClo Level 1 RFP reporter with AE fusion sites
BBa_K584011BBa_K584011 Version 1 (Component)Lac-Lux hybrid promotor + CrtEBI + CI repressor + INP
BBa_I741016BBa_I741016 Version 1 (Component)Reverse Total XylR Transcriptional Regulator Left Facing (I741011 reverse complement
BBa_K1968014BBa_K1968014 Version 1 (Component)Tcyc Phytobrick: cytochrome C gene transcriptional terminator from Saccharomyces cerevisiae
BBa_K611017BBa_K611017 Version 1 (Component)cI Lambda Repressor and Promoter Wild Type Control
BBa_K607037BBa_K607037 Version 1 (Component)pBAD/AraC generating cI transcription factor
BBa_I759033BBa_I759033 Version 1 (Component)cis1-repressed, tet-regulated YFP
BBa_I759045BBa_I759045 Version 1 (Component)cis7-repressed, tet-regulated YFP
BBa_I759043BBa_I759043 Version 1 (Component)cis6-repressed, tet-regulated YFP
BBa_I759037BBa_I759037 Version 1 (Component)cis3-repressed, tet-regulated YFP
BBa_I759047BBa_I759047 Version 1 (Component)cis8-repressed, tet-regulated YFP
BBa_I759041BBa_I759041 Version 1 (Component)cis5-repressed, tet-regulated YFP
BBa_I759035BBa_I759035 Version 1 (Component)cis2-repressed, tet-regulated YFP
BBa_K327015BBa_K327015 Version 1 (Component)Lux activated, C1lam repressed switch
BBa_K165100BBa_K165100 Version 1 (Component)Gli1 bs + LexA bs + mCYC + LexA repressor (mCherryx2 tagged) on pRS304*
BBa_K2092004BBa_K2092004 Version 1 (Component)alcR (incl RBS), ethanol-activated transcription factor from A. nidulans
BBa_K165101BBa_K165101 Version 1 (Component)Zif268-HIV bs + LexA bs + mCYC + Zif268-HIV repressor (mCherryx2 tagged) on pRS304*
BBa_K395102BBa_K395102 Version 1 (Component)GFP reporter repressed by LuxR and 3OC6HSL (K395005:K121013)
BBa_K395103BBa_K395103 Version 1 (Component)GFP reporter repressed by LuxR and 3OC6HSL (K395006:K121013)
BBa_K079051BBa_K079051 Version 1 (Component)LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac 1 operator
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.