FBS-AceB+L
BBa_K1163999 Version 1 (Component)
Inverter composed of Fur Binding site from AceB promoter + LacI-LVA

BBa_K2123112
BBa_K2123112 Version 1 (Component)
Tac promoter in tandem (3 repetition) with downstream mer operator + RFP (K081014)

BBa_K2123115
BBa_K2123115 Version 1 (Component)
Universal promoter (Tac + JK26) for both growth phase with downstream mer operator + K081014

BBa_K1113411
BBa_K1113411 Version 1 (Component)
Targeting sequence for the delivery of the LacZ gene to the Carboxysome

BBa_K079021
BBa_K079021 Version 1 (Component)
LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La

Adapter Bi
BBa_K1807015 Version 1 (Component)
This device allows for the IPTG-inducible expression of lacZα peptide which in the presence of

BBa_K137033
BBa_K137033 Version 1 (Component)
Device with GFP with (AC)21 repeat after start codon

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.