BBa_K1151030BBa_K1151030 Version 1 (Component)pint-RBS-GFP-TER-pUreA-RBS-RFP (LVAtag)
BBa_K1151028BBa_K1151028 Version 1 (Component)pexbB-RBS-GFP-TER-pUreA-RBS-RFP (LVAtag)
BBa_S03644BBa_S03644 Version 1 (Component)RFP<sub>rev</sub> : RBS<sub>rev</sub>
BBa_K1151029BBa_K1151029 Version 1 (Component)pnikR-RBS-GFP-TER-pUreA-RBS-RFP (LVAtag)
BBa_K101013BBa_K101013 Version 1 (Component)Construct with TetR/p22cII promoter, RBS, RFP, Terminator
BBa_K2123113BBa_K2123113 Version 1 (Component)Stationary phase promoter with downstream mer operator + RFP (K081014)
BBa_J162001BBa_J162001 Version 1 (Component)RBS cl RBS RFP RBS 3OC6HSL Double Terminator
BBa_K101012BBa_K101012 Version 1 (Component)Construct with TetR/p22cII promoter, RBS, RFP, Terminator
BBa_J01065BBa_J01065 Version 1 (Component)TEST R Mobilizable Plasmid with RFP reporter = [OriTR][OnRFP]
SETBBa_K1433011 Version 1 (Component)Terminator-RFP-RBS-attB-P-attP-RBS-GFP-Terminator
BBa_S03737BBa_S03737 Version 1 (Component)pLac-lox-RFP(reverse)-TT-lox-RBS-Tet (psB1A2)
BBa_K092500BBa_K092500 Version 1 (Component)TetR cds with RBS and Terminator + pTetR, RFP with RBS
BBa_K2123112BBa_K2123112 Version 1 (Component)Tac promoter in tandem (3 repetition) with downstream mer operator + RFP (K081014)
BBa_K1067000BBa_K1067000 Version 1 (Component)Periplasmic directed GFP SF with signal peptide TAT and RFP as background color
BBa_J22121BBa_J22121 Version 1 (Component)Lac Y gene under the rec A(SOS) promoter in plasmid pSB2K3
BBa_K2123114BBa_K2123114 Version 1 (Component)Stationary phase promoter in tandem (3 repetition) with downstream mer operator + RFP (K081014)
GG100BBa_K2145125 Version 1 (Component)This part contains 2 fluorescent protein coding sites (RFP and GFP) with a spacer
GG98BBa_K2145123 Version 1 (Component)This part contains 2 fluorescent protein coding sites (RFP and GFP) with a spacer
BBa_K2123117BBa_K2123117 Version 1 (Component)Novel RFP device regulated by mercury: MerR (regulatory protein) + Stationary phase with mer operato
BBa_K2123116BBa_K2123116 Version 1 (Component)Universal promoter for both phase of growth in tandem with downstram mer operator + RFP (K081014)
BBa_K1520509BBa_K1520509 Version 1 (Component)PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.