BBa_K2082252
BBa_K2082252 Version 1 (Component)
RFP under the control of an optimized lacZ promoter with lambda cI binding site combined with SH2:cI

BBa_K110023
BBa_K110023 Version 1 (Component)
YemCitrine Yeast Optimized (backwards)

BBa_K1845001
BBa_K1845001 Version 1 (Component)
Miraculin (Yeast codon optimised + His-tag)

BBa_K883705
BBa_K883705 Version 1 (Component)
IPTG induced promoter + GFP with a His-tag fused to a coiled coil

Venus YFP
BBa_J63001 Version 1 (Component)
enhanced version of EYFP, yeast-optimized YFP

BBa_K1695013
BBa_K1695013 Version 1 (Component)
Riboswitch + Bacteriophage 21 codon optimized R gene

BBa_K1695014
BBa_K1695014 Version 1 (Component)
Riboswitch + Bacteriophage 21 codon optimized Rz gene

BBa_K1695012
BBa_K1695012 Version 1 (Component)
Riboswitch + Bacteriophage 21 codon optimized S gene

BBa_K299124
BBa_K299124 Version 1 (Component)
[J23100].[B0034].[ E2050 mOrrange]

mCherry
BBa_K105010 Version 1 (Component)
mCherry, Yeast optimized for fusion proteins

BBa_K105008
BBa_K105008 Version 1 (Component)
EYFP, yeast optimized for fusion proteins

BBa_K1189030
BBa_K1189030 Version 1 (Component)
TALE-B with a his6 tag linked to a K coil under an inducible lacI promoter

BBa_K1441013
BBa_K1441013 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag INSERT

BBa_K1189029
BBa_K1189029 Version 1 (Component)
TALE-A with a his tag linked to a K coil under the control of a LacI promoter

BBa_K2152003
BBa_K2152003 Version 1 (Component)
Bacteriophage Phi X 174 lysis gene E(wild type)

BBa_K1695042
BBa_K1695042 Version 1 (Component)
Riboswitch Bacteriophage 21 Codon Optimized Lysis Cassette S R Rz

BBa_K1441012
BBa_K1441012 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag In pGAPz alpha A

BBa_K1695049
BBa_K1695049 Version 1 (Component)
pL8-UV5 + Riboswitch Bacteriophage 21 Codon Optimized Lysis Cassette S R Rz

PrtDEF
BBa_K258007 Version 1 (Component)
Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi

BBa_K831011
BBa_K831011 Version 1 (Component)
istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12

BBa_K831012
BBa_K831012 Version 1 (Component)
istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.