BBa_K1072022BBa_K1072022 Version 1 (Component)Lux pI+RBS+LuxR+2TM+Lux pR+RBS+LuxI+2TM+Lux pR+RBS+GFP+2TM+Lux pR+RBS+Ndh+2TM
BBa_J44010BBa_J44010 Version 1 (Component)HixC-pBAD-HixC-RBS-TetF-HixC
BBa_I715068BBa_I715068 Version 1 (Component)Partially flippable reverse Tet.
BBa_K415005BBa_K415005 Version 1 (Component)pLux/cI-OR : RBS-mCherry : Term : p(tetR) : RBS-luxR : Term
StalkerBBa_J06001 Version 1 (Component)Tet repressible luxI generator ( LVA+ )
BBa_K329003BBa_K329003 Version 1 (Component)Strong RBS (B0034) - Tetracycline resistance protein TetA(C) (J31007)
BBa_S03641BBa_S03641 Version 1 (Component)HixC : TetB-RBS<sub>rev</sub>-HixC
BBa_J06101BBa_J06101 Version 1 (Component)Tet repressible lasI generator ( LVA+ )
BBa_I759033BBa_I759033 Version 1 (Component)cis1-repressed, tet-regulated YFP
BBa_I759045BBa_I759045 Version 1 (Component)cis7-repressed, tet-regulated YFP
BBa_I759043BBa_I759043 Version 1 (Component)cis6-repressed, tet-regulated YFP
BBa_I759037BBa_I759037 Version 1 (Component)cis3-repressed, tet-regulated YFP
BBa_I759047BBa_I759047 Version 1 (Component)cis8-repressed, tet-regulated YFP
BBa_I759041BBa_I759041 Version 1 (Component)cis5-repressed, tet-regulated YFP
BBa_I759035BBa_I759035 Version 1 (Component)cis2-repressed, tet-regulated YFP
BBa_K1444011BBa_K1444011 Version 1 (Component)Composite promoter and consensus B. subtilis RBS - pTetR
BBa_S03638BBa_S03638 Version 1 (Component)HixC-pBad<sub>rev</sub>-HixC : RBS-TetF
BBa_S03736BBa_S03736 Version 1 (Component)pLac-lox-RBS-Tet (in pSB1A2)
BBa_K208013BBa_K208013 Version 1 (Component)Tet Repressible Promoter (BBa_R0040) and RBS (BBa_B0034)
BBa_K1444014BBa_K1444014 Version 1 (Component)Composite promoter and weak B. subtilis RBS - pTetR x2
BBa_S03766BBa_S03766 Version 1 (Component)RBS-Kan-RBS-Tet-RBS-RFP (pSB1A7)
BBa_S03674BBa_S03674 Version 1 (Component)(-1,2) HixC-pBad<sub>rev</sub>-HixC : RBS-TetF-HixC
BBa_K196013BBa_K196013 Version 1 (Component)Hybrid promoter (Lux cassette + c2 P22 promoter) + RBS + LuxR + ter
BBa_S03737BBa_S03737 Version 1 (Component)pLac-lox-RFP(reverse)-TT-lox-RBS-Tet (psB1A2)
BBa_K415011BBa_K415011 Version 1 (Component)PtetR : RBS : LuxR : Term : PluxR/cI-OR : RBS : mCherry : Term : Plux/cI-OR : RBS : LuxI
BBa_R4030BBa_R4030 Version 1 (Component)PoPS/RiPS Generator composed of the Tet promoter and a strong RBS (R0040.E0030)
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.