BBa_K2173000BBa_K2173000 Version 1 (Component)aiiA with degradation tag expressed under Plux-lambda hybrid promoter and a double terminator + mRFP
BBa_K137033BBa_K137033 Version 1 (Component)Device with GFP with (AC)21 repeat after start codon
pCMV-ECFP-BBa_I763023 Version 1 (Component)LacI coding device with ECFP as a reporter regulated by pCMV
BBa_K1834846BBa_K1834846 Version 1 (Component)Xylene Sensor! With AmilCP!
BBa_K1189029BBa_K1189029 Version 1 (Component)TALE-A with a his tag linked to a K coil under the control of a LacI promoter
BBa_K1361003BBa_K1361003 Version 1 (Component)Curli Fiber generator under the control of T7 promoter with a relatively strong expression of CsgA,C
BBa_K1148000BBa_K1148000 Version 1 (Component)CrtE under PhlbA
BBa_K1361001BBa_K1361001 Version 1 (Component)Curli Fiber generator under the control of T7 promoter with a relatively weak expression of CsgA,C
BBa_J04431BBa_J04431 Version 1 (Component)GFP Coding Device with promoter, RBS, GFP with LVA tag, and Terminator
BBa_K1361007BBa_K1361007 Version 1 (Component)Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag at a relative
BBa_K2082252BBa_K2082252 Version 1 (Component)RFP under the control of an optimized lacZ promoter with lambda cI binding site combined with SH2:cI
BBa_K1179005BBa_K1179005 Version 1 (Component)(Acyl-TyA-rtTA3-His) A membrane/exosome targeting rtTA3 fusion to send through exosomes with His tag
MLSBBa_K1119001 Version 1 (Component)Mitochondrial Leader Sequence with RFC25 standard
BBa_K2123117BBa_K2123117 Version 1 (Component)Novel RFP device regulated by mercury: MerR (regulatory protein) + Stationary phase with mer operato
BBa_K1463807BBa_K1463807 Version 1 (Component)RFP under J23112 promoter
BBa_K1059016BBa_K1059016 Version 1 (Component)GFP-LVA under J23101 control
BBa_K936012BBa_K936012 Version 1 (Component)Leader sequence that brings protein to periplasm
Adapter BiBBa_K1807015 Version 1 (Component)This device allows for the IPTG-inducible expression of lacZα peptide which in the presence of
BBa_J40001BBa_J40001 Version 1 (Component)LacI (LVA) + ECFP (LVA) under luxpR promoter (LuxR/HSL regulated)
BBa_K1463771BBa_K1463771 Version 1 (Component)MotA and MotB under J23112 promoter
BBa_K639004BBa_K639004 Version 1 (Component)rrnB P1-LacI-pLac-mCherry plausible stress sensor
BBa_K1323019BBa_K1323019 Version 1 (Component)Hfq expression cassette under a xylose inducible promoter
BBa_K112228BBa_K112228 Version 1 (Component){pelB>} The pelB leader without stop codon, BBb format
BBa_I766223BBa_I766223 Version 1 (Component)HMTM1-EE under weak constitutive promoter
BBa_K1463753BBa_K1463753 Version 1 (Component)MotB and B0032 RBS under J23103 promoter
BBa_K1463703BBa_K1463703 Version 1 (Component)MotA and B0032 RBS under J23103 promoter
BBa_K116503BBa_K116503 Version 1 (Component)GFP under control of pOmpC promoter.R0082+E0240
BBa_K1541025BBa_K1541025 Version 1 (Component)sfGFP under promoter P(Lux) with riboregulator RR12y
BBa_K077041BBa_K077041 Version 1 (Component)AiiA and cII under control of plac promotor
BBa_K145112BBa_K145112 Version 1 (Component)cI under T7 and PR<sub>R</sub> dual promotor
BBa_J22121BBa_J22121 Version 1 (Component)Lac Y gene under the rec A(SOS) promoter in plasmid pSB2K3
BBa_K323164BBa_K323164 Version 1 (Component)VioA and VioB enzymes fused with zinc fingers under pBAD promoter in pSB4K5
BBa_K323163BBa_K323163 Version 1 (Component)VioC, VioD and VioE enzymes fused with zinc fingers under pBAD promoter in pSB4C5
iGEM Parts Registryigem_collection Version 1 (Collection)The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.