BBa_K575009BBa_K575009 Version 1 (Component)LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP
BBa_K575011BBa_K575011 Version 1 (Component)LasR/PAI1 Inducible Promoter + RBS (B0034) + RFP
BBa_K575013BBa_K575013 Version 1 (Component)RhlR/PAI2 Inducible Promoter + RBS (B0034) + GFP
BBa_K575015BBa_K575015 Version 1 (Component)RhlR/PAI2 Inducible Promoter + RBS (B0034) + RFP
BBa_K607037BBa_K607037 Version 1 (Component)pBAD/AraC generating cI transcription factor
BBa_K545103BBa_K545103 Version 1 (Component)MerR (Mercury-Response transcriptional factor)
BBa_K545003BBa_K545003 Version 1 (Component)LacI repressor followed by pLac promoter regulating MerR and CinR
BBa_K575025BBa_K575025 Version 1 (Component)LasR/PAI1 Inducible Promoter + RBS (B0030) + GFP, Constitutive Promoter + RBS (B0034) + LasR
CFPBBa_K566004 Version 1 (Component)CFP protein generator without transcription terminator
BBa_K575027BBa_K575027 Version 1 (Component)LasR/PAI1 Inducible Promoter + RBS (B0030) + RFP, Constitutive Promoter + RBS (B0034) + LasR
BBa_K575028BBa_K575028 Version 1 (Component)LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP, Constitutive Promoter + RBS (B0030) + LasR
BBa_K575029BBa_K575029 Version 1 (Component)LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP, Constitutive Promoter + RBS (B0034) + LasR
BBa_K575030BBa_K575030 Version 1 (Component)LasR/PAI1 Inducible Promoter + RBS (B0034) + RFP, Constitutive Promoter + RBS (B0030) + LasR
BBa_K575031BBa_K575031 Version 1 (Component)LasR/PAI1 Inducible Promoter + RBS (B0034) + RFP, Constitutive Promoter + RBS (B0034) + LasR
BBa_K575033BBa_K575033 Version 1 (Component)RhlR/PAI2 Inducible Promoter + RBS (B0030) + GFP + Constitutive Promoter + RBS (B0034) + RhlR
BBa_K575035BBa_K575035 Version 1 (Component)RhlR/PAI2 Inducible Promoter + RBS (B0030) + RFP + Constitutive Promoter + RBS (B0034) + RhlR
BBa_K575036BBa_K575036 Version 1 (Component)RhlR/PAI2 Inducible Promoter + RBS (B0034) + GFP + Constitutive Promoter + RBS (B0030) + RhlR
BBa_K575037BBa_K575037 Version 1 (Component)RhlR/PAI2 Inducible Promoter + RBS (B0034) + GFP + Constitutive Promoter + RBS (B0034) + RhlR
BBa_K575038BBa_K575038 Version 1 (Component)RhlR/PAI2 Inducible Promoter + RBS (B0034) + RFP + Constitutive Promoter + RBS (B0030) + RhlR
BBa_K575039BBa_K575039 Version 1 (Component)RhlR/PAI2 Inducible Promoter + RBS (B0034) + RFP + Constitutive Promoter + RBS (B0034) + RhlR
BBa_K575040BBa_K575040 Version 1 (Component)Genomic LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP
BBa_K575041BBa_K575041 Version 1 (Component)Genomic LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP
BBa_K575042BBa_K575042 Version 1 (Component)Genomic RhlR/PAI2 Inducible Promoter + RBS (B0034) + GFP
BBa_K575044BBa_K575044 Version 1 (Component)Genomic LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP, Cons. Promoter + RBS (B0030) + LasR
BBa_K575045BBa_K575045 Version 1 (Component)Genomic LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP, Cons. Promoter + RBS (B0030) + LasR
BBa_K575046BBa_K575046 Version 1 (Component)Genomic RhlR/PAI2 Inducible Promoter + RBS (B0034) + GFP, Cons. Promoter + RBS (B0034) + RhlR
BBa_K575047BBa_K575047 Version 1 (Component)Genomic RhlR/PAI2 Inducible Promoter + RBS (B0030) + GFP, Cons. Promoter + RBS (B0034) + RhlR
BBa_K887070BBa_K887070 Version 1 (Component)Plac(promoter)+aRBS(B0034)+zif268+alsS+a RBS(B0034)+PBSII+ilvC
BBa_K887071BBa_K887071 Version 1 (Component)Plac(promoter)+aRBS(B0034)+zif268+alsS+a RBS(B0034)+PBSII+ilvC
BBa_K733002BBa_K733002 Version 1 (Component)<i>xylR</i>+<i>PxylA</i>: A xylose inducible promoter with its transcriptional regulator.
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
BBa_K759007BBa_K759007 Version 1 (Component)RBS(B0034)and phaB (acetoacetyl-CoA reductase orgin from Ralstonia eutropha)
BBa_K156027BBa_K156027 Version 1 (Component)Genetic attenuator (synthetic transcription terminator) ~68% efficient
BBa_K156029BBa_K156029 Version 1 (Component)Genetic attenuator (synthetic transcription terminator) ~17% efficient
BBa_M11082BBa_M11082 Version 1 (Component)ZntR:Zn(II)-responsive regulator of zntA (also MerR transcriptional regulator)
BBa_K228803BBa_K228803 Version 1 (Component)RBS(B0034)+lacI(C0012)
BBa_K228809BBa_K228809 Version 1 (Component)RBS(B0034)+tetR(C0040)
BBa_K228813BBa_K228813 Version 1 (Component)RBS(B0034)+lacI(C0012)+terminator(B0015)
BBa_K228814BBa_K228814 Version 1 (Component)RBS(B0034)+tetR(C0040)+terminator(B0015)
BBa_K228815BBa_K228815 Version 1 (Component)Promoter(constitutive)+RBS(B0034)+lacI(C0012)+terminator(B0015)
BBa_K228816BBa_K228816 Version 1 (Component)Promoter(constitutive)+RBS(B0034)+tetR(C0040)+terminator(B0015)
BBa_K228817BBa_K228817 Version 1 (Component)Pcat+RBS(B0034)+lacI(C0012)+terminator(B0015)+lacP(R0010)
BBa_K228818BBa_K228818 Version 1 (Component)Pcat+RBS(B0034)+tetR(C0040)+terminator(B0015)+tetP(R0040)
BBa_K228819BBa_K228819 Version 1 (Component)Pcat+RBS(B0034)+lacI(C0012)+term(B0015)+lacP(R0010)+GFP
BBa_K228820BBa_K228820 Version 1 (Component)Pcat+RBS(B0034)+tetR(C0040)+term(B0015)+tetP(R0040)+GFP
BBa_K228831BBa_K228831 Version 1 (Component)AND GATE lacP+SupD+AraC+RBS(B0034)+T7ptag
BBa_K228103BBa_K228103 Version 1 (Component)LacP(R0010) + RBS(B0034) + T7ptag(K228000) + terminator(B0015)
BBa_K228259BBa_K228259 Version 1 (Component)AND GATE AraC+SupD+Sal+RBS(B0034)+T7ptag
BBa_K228115BBa_K228115 Version 1 (Component)AND GATE AraC + SupD + LacP +RBS(B0034)+T7ptag