BBa_K101012BBa_K101012 Version 1 (Component)Construct with TetR/p22cII promoter, RBS, RFP, Terminator
BBa_K1441012BBa_K1441012 Version 1 (Component)DNA ligase from Escherichia coli with His-tag In pGAPz alpha A
BBa_K411123BBa_K411123 Version 1 (Component)Split RFP-eIF4A_A Generator (R0040 + B0034 + RFP_split_A+Linker+eIF4A_split_A + B0015)
BBa_K199071BBa_K199071 Version 1 (Component)I13453:K199014: Pbad promomtor with the suppressor tRNA of the codon AGGAC
SETBBa_K1433011 Version 1 (Component)Terminator-RFP-RBS-attB-P-attP-RBS-GFP-Terminator
BBa_S03737BBa_S03737 Version 1 (Component)pLac-lox-RFP(reverse)-TT-lox-RBS-Tet (psB1A2)
BBa_K092500BBa_K092500 Version 1 (Component)TetR cds with RBS and Terminator + pTetR, RFP with RBS
BBa_K2123112BBa_K2123112 Version 1 (Component)Tac promoter in tandem (3 repetition) with downstream mer operator + RFP (K081014)
BBa_K1067000BBa_K1067000 Version 1 (Component)Periplasmic directed GFP SF with signal peptide TAT and RFP as background color
BBa_K2123114BBa_K2123114 Version 1 (Component)Stationary phase promoter in tandem (3 repetition) with downstream mer operator + RFP (K081014)
GG100BBa_K2145125 Version 1 (Component)This part contains 2 fluorescent protein coding sites (RFP and GFP) with a spacer
GG98BBa_K2145123 Version 1 (Component)This part contains 2 fluorescent protein coding sites (RFP and GFP) with a spacer
BBa_K2123117BBa_K2123117 Version 1 (Component)Novel RFP device regulated by mercury: MerR (regulatory protein) + Stationary phase with mer operato
BBa_K2123116BBa_K2123116 Version 1 (Component)Universal promoter for both phase of growth in tandem with downstram mer operator + RFP (K081014)
BBa_K1520509BBa_K1520509 Version 1 (Component)PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.