BBa_K831014
BBa_K831014 Version 1 (Component)
Inducible istR (inhibitor of SOS-induced toxicity by RNA) under the control of lac promoter

BBa_K539691
BBa_K539691 Version 1 (Component)
promoter(lacI regulated)+Alss+ilvC+ilvD(each preceded by own RBS)and RNA thermometer+terminator

BBa_I9001
BBa_I9001 Version 1 (Component)
QPI (mnt) B0034.C0073.B0015.R0073

BBa_J31016
BBa_J31016 Version 1 (Component)
part produces the RNA construct crRNA-RBS-GFPLVA-tt that can only be translated in the presence of t

BBa_K1132000
BBa_K1132000 Version 1 (Component)
T7 RNA Polymerase

BBa_K1100076
BBa_K1100076 Version 1 (Component)
RNA-IN S49

BBa_K1864001
BBa_K1864001 Version 1 (Component)
T7 RNA Polymerase

BBa_K1942001
BBa_K1942001 Version 1 (Component)
This part is a short RNA sequence designed for KRAS gene silencing. It is used for down-regulating K

RNAcj
BBa_K1826005 Version 1 (Component)
RNA thermometer + cjBlue

BBa_K189060
BBa_K189060 Version 1 (Component)
Gp15 of Bacteriophage Mu

BBa_K1777012
BBa_K1777012 Version 1 (Component)
sequence-specific RNA binding protein

BBa_K1777014
BBa_K1777014 Version 1 (Component)
sequence-specific RNA binding protein

BBa_K1777013
BBa_K1777013 Version 1 (Component)
sequence-specific RNA binding protein

BBa_K1777004
BBa_K1777004 Version 1 (Component)
sequence-specific RNA binding protein

BBa_K2050419
BBa_K2050419 Version 1 (Component)
RNA Polimerase III Promoter (H1 promoter)

BBa_K1824559
BBa_K1824559 Version 1 (Component)
RNA Thermometer FourU (Specially designed for J23119)

BBa_K1824558
BBa_K1824558 Version 1 (Component)
RNA Thermometer A1 (Specially designed for T7)

BBa_J92006
BBa_J92006 Version 1 (Component)
M. Ruminantium RNA polymerase Subunit A"

BBa_K2050422
BBa_K2050422 Version 1 (Component)
Mouse H1 promoter (RNA Polymerase III promoter)

BBa_K1363604
BBa_K1363604 Version 1 (Component)
key of no of RNA logic gates

BBa_K809710
BBa_K809710 Version 1 (Component)
GAL promoter + kozak + ZIM17 + T7 RNAP + ADH1 terminator

BBa_K1014015
BBa_K1014015 Version 1 (Component)
Biological proportional operational Mu-circuit(1)

BBa_K581003
BBa_K581003 Version 1 (Component)
SgrS2+Terminator (small RNA regulator, conjugate part of ptsG2)

BBa_J24823
BBa_J24823 Version 1 (Component)
same as J24819 but with ACCACC Euk RBS removed and problem solved via J24822 removed

BBa_K779120
BBa_K779120 Version 1 (Component)
RNA Reporter top strand with quencher (RQ) and tag fluorophore (Alexa 488) MammoBlock

BBa_K831011
BBa_K831011 Version 1 (Component)
istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12

BBa_K831012
BBa_K831012 Version 1 (Component)
istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.