RNAcj

BBa_K1826005 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K1826005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Saishreyas Gourishankar Iyer
Date created: 2016-10-04 11:00:00
Date modified: 2016-10-05 06:55:11

RNA thermometer + cjBlue



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1826005_sequence (Version 1)

Description

This part is a composite of RNA thermometer and cjBlue, green chromoprotein. The RNA thermometer is an untranslated RNA sequence that will allow translation only above 42??C. Below this temperature, the RNA thermometer sequence will fold into a complex structure and prevent translation. The cjBlue , green chromoprotein is from the Cnidopus japonicus sea anemone. It naturally exhibits dark green color when expressed. The cjBlue protein is readily expressed in E.coli. The cjBlue acts as a reporter gene and the RNA thermometer, as a temperature controlled switch.

Notes

Since the RNA thermometer sequence was too small, we had to keep it in its backbone and ligate cjBlue to it.

Source

The cjBlue , green chromoprotein is from the Cnidopus japonicus sea anemone. RNA thermometer is from Bradyrhizobium japonicum that initiates translation at 42??C.

Access Instance Definition
public
public
BBa_K115001
BBa_K592011
BBa_K115001
cjBlue
igem#experience
None
 
igem#status
Deleted
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1826005/1