BBa_I13563
BBa_I13563 Version 1 (Component)
BioBrick site flanked by RNase sites and hairpins for use in Screening Plasmids

iGEM 2019 Distribution
iGEM2019Distribution_collection Version 1 (Collection)
Distribution of parts for the 2019 iGEM competition

iGEM 2018 Distribution
iGEM2018Distribution_collection Version 1 (Collection)
Distribution of parts for the 2018 iGEM competition

BBa_K1039005
BBa_K1039005 Version 1 (Component)
Bxb1 Invertible Promoter Switch (Promoter J23118) RL State

BBa_K1039004
BBa_K1039004 Version 1 (Component)
Bxb1 Invertible Promoter Switch (Promoter J23119) (RL State)

BBa_K1039010
BBa_K1039010 Version 1 (Component)
PhiC31 Invertible Promoter Switch (Promoter J23119) ??? RL State

BBa_K1039011
BBa_K1039011 Version 1 (Component)
PhiC31 Invertible Promoter Switch (Promoter J23118) ??? RL State

RFY_SPfer
BBa_K809302 Version 1 (Component)
RFP + signal peptide of FER

YFP_SPfer
BBa_K809314 Version 1 (Component)
YFP + signal peptide of FER

PLac-CI-La
BBa_I763019 Version 1 (Component)
CI and LacY coding device switched on by IPTG with GFP as a reporter

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_M36733
BBa_M36733 Version 1 (Component)
Fur promoter + Gemini

KWR
BBa_K175030 Version 1 (Component)
Key for lock of weak (K175029) RBS (B0031) from the lock/key library TUD09

BBa_I735010
BBa_I735010 Version 1 (Component)
This promoter encoding for a thiolase involved in beta-oxidation of fatty acids.

BBa_K175046
BBa_K175046 Version 1 (Component)
Plasmid 1 for Transcriptional Cascade subproject TU Delft iGEM 09

BBa_K309021
BBa_K309021 Version 1 (Component)
pStr-220::eCFP::3'UTR (for use in C. elegans)

BBa_I12056
BBa_I12056 Version 1 (Component)
A protein (lambda cI) subassembly for modified BL oscillator based on I12036

BBa_I12055
BBa_I12055 Version 1 (Component)
A protein (lambda cI) subassembly for modified BL oscillator based on I12006

BBa_I12057
BBa_I12057 Version 1 (Component)
A protein (lambda cI) subassembly for modified BL oscillator based on I12040

BBa_K801040
BBa_K801040 Version 1 (Component)
SV40NLS-PhyB-linker-Gal4DBD (part for GAL4 based light switchable promoter system)

BBa_K1150026
BBa_K1150026 Version 1 (Component)
uniCAS Red Light Switch Part II - Activator

BBa_K1150030
BBa_K1150030 Version 1 (Component)
uniCAS UV Light Switch Part II - Activator

BBa_K1150027
BBa_K1150027 Version 1 (Component)
uniCAS Red Light Switch Part II - Repressor

BBa_K1150031
BBa_K1150031 Version 1 (Component)
uniCAS UV Light Switch Part II - Repressor

BBa_K163001
BBa_K163001 Version 1 (Component)
Mussel adhesive protein from Mytilus Edulis (Mefp-5) optimized for Caulobacter

BBa_K363003
BBa_K363003 Version 1 (Component)
A calcium dependent response element binding site for the Crz1 activator

BBa_J119313
BBa_J119313 Version 1 (Component)
pClone Blue - Device for Testing New Promoters via Golden Gate Assembly

BBa_I758601
BBa_I758601 Version 1 (Component)
Screen for binding affinity of mutant cI lambda to promotor sites

BBa_K363005
BBa_K363005 Version 1 (Component)
A calcium dependent response element binding site for the Crz1 activator

BBa_K363007
BBa_K363007 Version 1 (Component)
A calcium dependent response element binding site for the Crz1 activator

Adapter Bi
BBa_K1807000 Version 1 (Component)
Protein generator device suitable for blue-white screening and Gibson Assembly.

BBa_K363004
BBa_K363004 Version 1 (Component)
A calcium dependent response element binding site for the Crz1 activator

BBa_J119137
BBa_J119137 Version 1 (Component)
pClone Red - Device for Testing New Promoters via Golden Gate Assembly

BBa_K1179000
BBa_K1179000 Version 1 (Component)
(Acyl-TyA) A membrane targeting domain for exosomal transportation of proteins

BBa_K201012
BBa_K201012 Version 1 (Component)
Trans-Repressor (7) sequence, to be used for T-REX device

BBa_K1351040
BBa_K1351040 Version 1 (Component)
pBS0K<i>Pspac</i>, an IPTG-inducible replicative expression vector for

BBa_K497018
BBa_K497018 Version 1 (Component)
Forward primer Deinococcus radiodurans bacteriophytochrome gene for insertion after heme oxygenase

BBa_K497019
BBa_K497019 Version 1 (Component)
Reverse primer Deinococcus radiodurans bacteriophytochrome gene for insertion after heme oxygenase

BBa_J95022
BBa_J95022 Version 1 (Component)
PA1/04/03 promoter used for gene expression in R. sphaeroids

BBa_K201011
BBa_K201011 Version 1 (Component)
Trans-Repressor (4) sequence, to be used for T-REX device

BBa_K363012
BBa_K363012 Version 1 (Component)
Cnb1, the calcium sensitive regulatory subunit for calcineurin from S. cerevisiae

BBa_I12227
BBa_I12227 Version 1 (Component)
Generator for TetR-4C and lambda cI - Gal4 fusion protein

T7
BBa_K145014 Version 1 (Component)
T7 RNA Polymerase with UmuD derived tag

BBa_K424017
BBa_K424017 Version 1 (Component)
Test plataform for rhamnosyltransferase BioBrick (Rh1AB_BB) expression in E. coli

BBa_K1368015
BBa_K1368015 Version 1 (Component)
Cre recombinase with post-transcriptional control driven by IPTG

BBa_K1150029
BBa_K1150029 Version 1 (Component)
uniCAS UV Light Switch Part I - Stimulator

BBa_K1150025
BBa_K1150025 Version 1 (Component)
uniCAS Red Light Switch Part I - Stimulator

pSBBs4S
BBa_K823024 Version 1 (Component)
pSB_Bs4S-P<i>_xyl</i>: Integrative expression vector for <i>Bacillus subtilis</i

BBa_K891002
BBa_K891002 Version 1 (Component)
Probe coding for alpha fragment of beta gal (long linker)

BBa_K891007
BBa_K891007 Version 1 (Component)
Probe coding for omega fragment of beta gal (longer linker)