BBa_I714062
BBa_I714062 Version 1 (Component)
[E0040][B0015] ([GFP][Double Terminator])

BBa_K195201
BBa_K195201 Version 1 (Component)
alpha-V integrin gene+terminator

BBa_K092000
BBa_K092000 Version 1 (Component)
TetR coding sequence with RBS and terminator

BBa_K1877001
BBa_K1877001 Version 1 (Component)
pBAD + RBS + GFP + Single Terminator

BBa_K1104931
BBa_K1104931 Version 1 (Component)
RBS+LuxI+RBS+LuxR+Terminator

BBa_K228814
BBa_K228814 Version 1 (Component)
RBS(B0034)+tetR(C0040)+terminator(B0015)

BBa_K944007
BBa_K944007 Version 1 (Component)
Higly engineered mutant of RFP and a double terminator

RBS-TetR-T
BBa_K079007 Version 1 (Component)
TetR protein with terminator and RBS

RBS-LacI-T
BBa_K079002 Version 1 (Component)
LacI protein with terminator and RBS

RBS-CI-T
BBa_K079010 Version 1 (Component)
CI protein with terminator and RBS

BBa_K228868
BBa_K228868 Version 1 (Component)
AraC(K228009) + RBS(B0033) + T7ptag(K228000) + terminator(B0015)

BBa_K228869
BBa_K228869 Version 1 (Component)
AraC(K228009) + RBS(B0031) + T7ptag(K228000) + terminator(B0015)

BBa_J72046
BBa_J72046 Version 1 (Component)
{rbs.GFP(E0040).double terminator} in BglBrick Format

mCherry
BBa_J06703 Version 1 (Component)
mCherry, bacterial (LVA+) with RBS and forward terminator

BBa_K1685002
BBa_K1685002 Version 1 (Component)
aeBlue with LVA tag and double terminator

BBa_K1660010
BBa_K1660010 Version 1 (Component)
RFP+RBS+attB+P+attP+RBS+GFP+Terminator

BBa_J95020
BBa_J95020 Version 1 (Component)
Terminator measurement kit designed for E coli

BBa_K581003
BBa_K581003 Version 1 (Component)
SgrS2+Terminator (small RNA regulator, conjugate part of ptsG2)

BBa_K809721
BBa_K809721 Version 1 (Component)
DLD3 promoter + kozak + hoiln(lambda phage) + ADH1 terminator

BBa_K195202
BBa_K195202 Version 1 (Component)
Lpp-OmpA-linker+alpha-V integrin gene+terminator

BBa_K584023
BBa_K584023 Version 1 (Component)
Ice Neucleating Protein Generator (RBS + InaZ [K584021] + Terminator)

BBa_K584022
BBa_K584022 Version 1 (Component)
Ice Neucleating Protein Generator (RBS + InaZ [K584021] + Terminator)

BBa_K101012
BBa_K101012 Version 1 (Component)
Construct with TetR/p22cII promoter, RBS, RFP, Terminator

BBa_K1968014
BBa_K1968014 Version 1 (Component)
Tcyc Phytobrick: cytochrome C gene transcriptional terminator from Saccharomyces cerevisiae

BBa_K195203
BBa_K195203 Version 1 (Component)
RBS+Lpp-OmpA-linker+alpha-V integrin gene+terminator

BBa_K861012
BBa_K861012 Version 1 (Component)
FadD and FadL with a RBS and a terminator

BBa_K332034
BBa_K332034 Version 1 (Component)
Constitutive promoter + 37℃ induced RBS + tetR + double terminator

BBa_K132016
BBa_K132016 Version 1 (Component)
luxI+KanR-LVA+LacI+PL+KanR-LVA+aiiA+terminator

BBa_K092500
BBa_K092500 Version 1 (Component)
TetR cds with RBS and Terminator + pTetR, RFP with RBS

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.