Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Haoqian Zhang
Date created: 2009-09-21 11:00:00
Date modified: 2015-05-08 01:11:34
AraC(K228009) + RBS(B0031) + T7ptag(K228000) + terminator(B0015)
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K228869_sequence (Version 1) |
Description
T7 polymerase with an amber mutation coding gene is constructed after AraC protein(reversed sequence) and Pbad promoter which would be triggered by Arabinose. T7 polymerase can work well in the exsitence of SupD tRNA. Besides, the expression of the T7 polymerase can be regulated by RBS.Notes
when cut this part for insert and ligate it into a vetor, the insert and vector may have the similar sizes which may disturb the ligation.Source
from 2009 part registry distribution & Peking University 2009 igem team| Access | Instance | Definition |
| public public public public public public | BBa_B0010 BBa_B0012 BBa_K228009 BBa_B0031 BBa_K228000 BBa_B0015 | BBa_B0010 BBa_B0012 BBa_K228009 BBa_B0031 BBa_K228000 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_B0010 BBa_B0012 BBa_K228009 BBa_B0031 BBa_K228000 BBa_B0015 | 1013,1092 1101,1141 1,1279 1288,1301 1308,3980 3989,4117 | BBa_B0010 BBa_B0012 BBa_K228009 BBa_B0031 BBa_K228000 BBa_B0015 |