BBa_K737001
BBa_K737001 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

BBa_K1676120
BBa_K1676120 Version 1 (Component)
Mutant 16 of Lactate Dehydrogenase

BBa_K1676114
BBa_K1676114 Version 1 (Component)
Mutant 10 of Lactate Dehydrogenase

BBa_K1361007
BBa_K1361007 Version 1 (Component)
Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag at a relative

BBa_K2082252
BBa_K2082252 Version 1 (Component)
RFP under the control of an optimized lacZ promoter with lambda cI binding site combined with SH2:cI

BBa_K1228001
BBa_K1228001 Version 1 (Component)
A fragment of loctoferrin

BBa_I759033
BBa_I759033 Version 1 (Component)
cis1-repressed, tet-regulated YFP

BBa_I759045
BBa_I759045 Version 1 (Component)
cis7-repressed, tet-regulated YFP

BBa_I759043
BBa_I759043 Version 1 (Component)
cis6-repressed, tet-regulated YFP

BBa_I759037
BBa_I759037 Version 1 (Component)
cis3-repressed, tet-regulated YFP

BBa_I759047
BBa_I759047 Version 1 (Component)
cis8-repressed, tet-regulated YFP

BBa_I759041
BBa_I759041 Version 1 (Component)
cis5-repressed, tet-regulated YFP

BBa_I759035
BBa_I759035 Version 1 (Component)
cis2-repressed, tet-regulated YFP

BBa_K2041010
BBa_K2041010 Version 1 (Component)
plac-RBS-Bxb1-T-plux-RBS-luxR-T-plux-recognition site of Bxb1-RBS-luxI-RBS-GFP-T

BBa_S03736
BBa_S03736 Version 1 (Component)
pLac-lox-RBS-Tet (in pSB1A2)

RFP_SPtim2
BBa_K809303 Version 1 (Component)
RFP + signal peptide of TIM21

BBa_K337088
BBa_K337088 Version 1 (Component)
Fragment 7 of wt AAV6

BBa_K337060
BBa_K337060 Version 1 (Component)
Fragment 3 of wt AAV1

BBa_K1413042
BBa_K1413042 Version 1 (Component)
OriVR6Kgamma origin of replication (ori) 2

BBa_S03766
BBa_S03766 Version 1 (Component)
RBS-Kan-RBS-Tet-RBS-RFP (pSB1A7)

T25 domain
BBa_K1088056 Version 1 (Component)
T25 domain of CyaA from Bordetella pertussis

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_M31786
BBa_M31786 Version 1 (Component)
1st half of Gene III- preBamHI cut

BBa_K648103
BBa_K648103 Version 1 (Component)
RecA with mutation of Arg 243

CsgD
BBa_K1019001 Version 1 (Component)
CsgD: positive regulator of curlin genes

BBa_K648102
BBa_K648102 Version 1 (Component)
RecA with mutation of Lys 286

BBa_K1114003
BBa_K1114003 Version 1 (Component)
The MoClo format of BBa_J23103 with AB fusion sites.

PrtDEF
BBa_K258007 Version 1 (Component)
Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi

BBa_K831011
BBa_K831011 Version 1 (Component)
istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12

BBa_K831012
BBa_K831012 Version 1 (Component)
istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12

BBa_I733005
BBa_I733005 Version 1 (Component)
Produce GFP in presence of AHL

BBa_K944007
BBa_K944007 Version 1 (Component)
Higly engineered mutant of RFP and a double terminator

BBa_K1725317
BBa_K1725317 Version 1 (Component)
Member of the RBS library - derived from BBa_B0032

BBa_J11009
BBa_J11009 Version 1 (Component)
variant of J11005 (low temp color) with stronger RBS

BBa_K1947017
BBa_K1947017 Version 1 (Component)
We verify the expression effect of Mms13.

BBa_J14462
BBa_J14462 Version 1 (Component)
Composite part comprised of J13002 and J04650

BBa_K1051262
BBa_K1051262 Version 1 (Component)
The measurement pathway of degradation tag K1051208.

BBa_K1051260
BBa_K1051260 Version 1 (Component)
The measurement pathway of degradation tag K1051206.

BBa_K1051261
BBa_K1051261 Version 1 (Component)
The measurement pathway of degradation tag K1051207.

BBa_K294125
BBa_K294125 Version 1 (Component)
A coding sequence for one of the heat shock protein:hchA

BBa_J14459
BBa_J14459 Version 1 (Component)
Composite part comprised of J04500 and J04630

BBa_J14464
BBa_J14464 Version 1 (Component)
Composite part comprised of J13002 and J04630

BBa_J14456
BBa_J14456 Version 1 (Component)
Composite part comprised of R0040 and E0422

BBa_K1676088
BBa_K1676088 Version 1 (Component)
Mutant 12 of LDH and wildtype LDP

BBa_K783037
BBa_K783037 Version 1 (Component)
This is a MoClo converted version of BBa_J23118

BBa_K783039
BBa_K783039 Version 1 (Component)
This is a MoClo converted version of BBa_J23101

BBa_K783049
BBa_K783049 Version 1 (Component)
This is a MoClo converted version of BBa_B0032

BBa_K783040
BBa_K783040 Version 1 (Component)
This is a MoClo converted version of BBa_J23110

BBa_K1413045
BBa_K1413045 Version 1 (Component)
A fusion of Universal Transposon Plasmid and pSB1C3