BBa_I12052
BBa_I12052 Version 1 (Component)
Test circuit for I12036

BBa_I12053
BBa_I12053 Version 1 (Component)
Test circuit for I12040

BBa_K1773015
BBa_K1773015 Version 1 (Component)
pLux/ci lambda promoter with 10bp at 3' end for RBS attachment

BBa_K1778005
BBa_K1778005 Version 1 (Component)
eGFP:enhanced Green Fluorescent Protein. It???s the mutant of GFP. It is widely used as report gene

BBa_J11009
BBa_J11009 Version 1 (Component)
variant of J11005 (low temp color) with stronger RBS

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_J06602
BBa_J06602 Version 1 (Component)
Construction Intermediate: mCherry, bacterial with RBS (B0034.J06504)

BBa_K1676082
BBa_K1676082 Version 1 (Component)
Mutant 6 of LDH and wildtype LDP

BBa_K1676078
BBa_K1676078 Version 1 (Component)
Mutant 2 of LDH and wildtype LDP

BBa_K1676083
BBa_K1676083 Version 1 (Component)
Mutant 7 of LDH and wildtype LDP

BBa_K1676094
BBa_K1676094 Version 1 (Component)
Mutant 18 of LDH and wildtype LDP

BBa_K1676095
BBa_K1676095 Version 1 (Component)
Mutant 19 of LDH and wildtype LDP

BBa_K1676085
BBa_K1676085 Version 1 (Component)
Mutant 9 of LDH and wildtype LDP

BBa_K1676087
BBa_K1676087 Version 1 (Component)
Mutant 11 of LDH and wildtype LDP

BBa_K1676092
BBa_K1676092 Version 1 (Component)
Mutant 16 of LDH and wildtype LDP

BBa_K1676084
BBa_K1676084 Version 1 (Component)
Mutant 8 of LDH and wildtype LDP

BBa_K1676090
BBa_K1676090 Version 1 (Component)
Mutant 14 of LDH and wildtype LDP

BBa_K1676093
BBa_K1676093 Version 1 (Component)
Mutant 17 of LDH and wildtype LDP

BBa_K1676089
BBa_K1676089 Version 1 (Component)
Mutant 13 of LDH and wildtype LDP

BBa_K1676077
BBa_K1676077 Version 1 (Component)
Mutant 1 of LDH and wildtype LDP

BBa_K1676079
BBa_K1676079 Version 1 (Component)
Mutant 3 of LDH and wildtype LDP

BBa_K1676081
BBa_K1676081 Version 1 (Component)
Mutant 5 of LDH and wildtype LDP

BBa_K415010
BBa_K415010 Version 1 (Component)
This is part K415006 with an appended Plux/cI promoter (R0065)

BBa_J58002
BBa_J58002 Version 1 (Component)
Robins Part for test purposes

BBa_J33009
BBa_J33009 Version 1 (Component)
this is just a test

BBa_K2018035
BBa_K2018035 Version 1 (Component)
phaCAB genes with additional promoter and RBS, that encodes proteins that can create PHB.

BBa_J01075
BBa_J01075 Version 1 (Component)
TEST [pTet][cI][DblTerminator]

BBa_K1001758
BBa_K1001758 Version 1 (Component)
AraC coding upstream of Lux promoter upstream of GFP

BBa_K208051
BBa_K208051 Version 1 (Component)
Tet Promoter/RBS/TorA/GFP/Terminator

BBa_J07067
BBa_J07067 Version 1 (Component)
FecI test construct w/ inducible promoter

BBa_P10306
BBa_P10306 Version 1 (Component)
tRFP

BBa_K777008
BBa_K777008 Version 1 (Component)
Tar receptor under the control of constitutive promoter J23114

BBa_K777002
BBa_K777002 Version 1 (Component)
Tar receptor under the control of constitutive promoter J23104

BBa_K777007
BBa_K777007 Version 1 (Component)
Tar receptor under the control of constitutive promoter J23113

BBa_K777005
BBa_K777005 Version 1 (Component)
Tar receptor under the control of constitutive promoter J23109

BBa_K777003
BBa_K777003 Version 1 (Component)
Tar receptor under the control of constitutive promoter J23105

BBa_K777006
BBa_K777006 Version 1 (Component)
Tar receptor under the control of constitutive promoter J23112

BBa_K777004
BBa_K777004 Version 1 (Component)
Tar receptor under the control of constitutive promoter J23106

BBa_K861051
BBa_K861051 Version 1 (Component)
FadR with a RBS and a terminator

BBa_K1065302
BBa_K1065302 Version 1 (Component)
Blue light sensing device without inverter for the production of amilGFP

BBa_K1179013
BBa_K1179013 Version 1 (Component)
eYFP (Yellow) fluorescent protein with a fused PEST site for rapid degradation

BBa_K777103
BBa_K777103 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23105

rbs+COMT
BBa_I742109 Version 1 (Component)
COMT gene with ribosome binding site

BBa_J70050
BBa_J70050 Version 1 (Component)
Left Library Insert Preparation Part (To use with J70052, and J70040)

BBa_I723124
BBa_I723124 Version 1 (Component)
Our test reporter, man!

BBa_K1657000
BBa_K1657000 Version 1 (Component)
It is called BAT. It have the resistance to glyphosate and the 2, 4-Dichlorophenoxyacetic acid

BBa_K1475007
BBa_K1475007 Version 1 (Component)
GFP controlled by lacI and plac

BBa_K1461231
BBa_K1461231 Version 1 (Component)
arabinose-induced GFP and taRNA generator

BBa_J107053
BBa_J107053 Version 1 (Component)
IPTG and 3OC6HSL inducible mRFP generator

BBa_K902015
BBa_K902015 Version 1 (Component)
MgtA riboswitch with GFP-LVA