BBa_S01821
BBa_S01821 Version 1 (Component)
Intermediate part from assembly 240

BBa_K750008
BBa_K750008 Version 1 (Component)
Quorum sensing system based on LuxI and LuxR to control the expression of parts behind

BBa_S02001
BBa_S02001 Version 1 (Component)
Intermediate part from assembly 240

BBa_M45101
BBa_M45101 Version 1 (Component)
Composite biobrick intended to induce biofilm formation in the presence of lactose. Made from parts

BBa_K2066084
BBa_K2066084 Version 1 (Component)
Promoter characterization part - J23103 without RiboJ

BBa_K2066063
BBa_K2066063 Version 1 (Component)
Promoter characterization part: J23103 with RiboJ

BBa_J14462
BBa_J14462 Version 1 (Component)
Composite part comprised of J13002 and J04650

BBa_K332024
BBa_K332024 Version 1 (Component)
A part of cell-cell-sigaling system

BBa_J14459
BBa_J14459 Version 1 (Component)
Composite part comprised of J04500 and J04630

BBa_J14464
BBa_J14464 Version 1 (Component)
Composite part comprised of J13002 and J04630

BBa_K564016
BBa_K564016 Version 1 (Component)
Upstream mutated chitoporin part fused with lacZ

BBa_K564017
BBa_K564017 Version 1 (Component)
Upstream mutated chitoporin part fused with lacZ

BBa_K581003
BBa_K581003 Version 1 (Component)
SgrS2+Terminator (small RNA regulator, conjugate part of ptsG2)

BBa_S03438
BBa_S03438 Version 1 (Component)
--Specify Parts List--

BBa_S01655
BBa_S01655 Version 1 (Component)
--Specify Parts List--

BBa_S01650
BBa_S01650 Version 1 (Component)
--Specify Parts List--

BBa_S01642
BBa_S01642 Version 1 (Component)
--Specify Parts List--

BBa_S01532
BBa_S01532 Version 1 (Component)
--Specify Parts List--

BBa_S03389
BBa_S03389 Version 1 (Component)
--Specify Parts List--

BBa_S03413
BBa_S03413 Version 1 (Component)
--Specify Parts List--

BBa_S01533
BBa_S01533 Version 1 (Component)
--Specify Parts List--

BBa_S01839
BBa_S01839 Version 1 (Component)
--Specify Parts List--

BBa_S01902
BBa_S01902 Version 1 (Component)
--Specify Parts List--

BBa_K1172914
BBa_K1172914 Version 1 (Component)
Part 2 of the Biosafety-System TetOR alive (TetO GFP)

BBa_J70084
BBa_J70084 Version 1 (Component)
Adds 6 his suffix, using BioScaffold part J70030 (PpiI) in pSB1AT3

[OriTR]+[R
BBa_K1439002 Version 1 (Component)
This part contains a reporter gene BBa_J04450, combined with OriTR. Used to test plasmid mobility.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.