BBa_K887003
BBa_K887003 Version 1 (Component)
Plac+B0034+alsS+zif268+B0034+PBS II+ilvC+B0034+HIVC+ilvD+Ptet+B0032+kivD+B0015

BBa_K165098
BBa_K165098 Version 1 (Component)
Zif268-HIV bs + ADH1 + Gli1 repressor (CFPx2 tagged) on pRS304*

BBa_K1462800
BBa_K1462800 Version 1 (Component)
GAL1+GBD+SH3+SH3+SH3+PDZ+PDZ+PDZ+PDZ+ADH1

BBa_K1462830
BBa_K1462830 Version 1 (Component)
GAL1+GBD+SH3+SH3+SH3+SH3+PDZ+PDZ+PDZ+ADH1

BBa_K1462660
BBa_K1462660 Version 1 (Component)
GAL1+GBD+SH3+SH3+SH3+SH3+PDZ+PDZ+Tom22+ADH1

BBa_K1462600
BBa_K1462600 Version 1 (Component)
GAL1+GBD+SH3+SH3+PDZ+PDZ+PDZ+PDZ+Tom22+ADH1

BBa_K1462630
BBa_K1462630 Version 1 (Component)
GAL1+GBD+SH3+SH3+SH3+PDZ+PDZ+PDZ+Tom22+ADH1

BBa_K165044
BBa_K165044 Version 1 (Component)
Zif268-HIV DNA binding domain with SV40 NLS and ADH1 terminator

BBa_M31513
BBa_M31513 Version 1 (Component)
Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III

BBa_M31516
BBa_M31516 Version 1 (Component)
Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III

BBa_K749010
BBa_K749010 Version 1 (Component)
Formate dehydrogenase device

BBa_K1462840
BBa_K1462840 Version 1 (Component)
GAL1+GBD+SH3+SH3+SH3+SH3+PDZ+PDZ+PDZ+PDZ+ADH1

BBa_K105025
BBa_K105025 Version 1 (Component)
Adh1 terminator

BBa_K1462670
BBa_K1462670 Version 1 (Component)
GAL1+GBD+SH3+SH3+SH3+SH3+PDZ+PDZ+PDZ+Tom22+ADH1

BBa_K1462640
BBa_K1462640 Version 1 (Component)
GAL1+GBD+SH3+SH3+SH3+PDZ+PDZ+PDZ+PDZ+Tom22+ADH1

BBa_K749013
BBa_K749013 Version 1 (Component)
Formate dehydrogenase device

BBa_K1453304
BBa_K1453304 Version 1 (Component)
pBluescript II KS(+)_5_copy

BBa_K1453303
BBa_K1453303 Version 1 (Component)
pBluescript II KS(+)_3_copy

BBa_K1676120
BBa_K1676120 Version 1 (Component)
Mutant 16 of Lactate Dehydrogenase

BBa_K1676114
BBa_K1676114 Version 1 (Component)
Mutant 10 of Lactate Dehydrogenase

BBa_K1462680
BBa_K1462680 Version 1 (Component)
GAL1+GBD+SH3+SH3+SH3+SH3+PDZ+PDZ+PDZ+PDZ+Tom22+ADH1

BBa_K1465304
BBa_K1465304 Version 1 (Component)
<i>adhA</i> gene from <i>Lactococcus lactis</i> under <i>T7</i> control

BBa_K1465305
BBa_K1465305 Version 1 (Component)
<i>adhA</i> gene from <i>Lactococcus lactis</i> under <i>Ptac</i> control

Strep-Tag
BBa_I757014 Version 1 (Component)
Strep-Tag II affinity tag

BBa_M31114
BBa_M31114 Version 1 (Component)
M13, gene II from HpaI to the end [1-831bp]

BBa_K809321
BBa_K809321 Version 1 (Component)
GAL promoter + YFP_SPtim21 + ADH1 terminator

BBa_K809308
BBa_K809308 Version 1 (Component)
GAL promoter + RFP_SPzim17 + ADH1 terminator

BBa_K809721
BBa_K809721 Version 1 (Component)
DLD3 promoter + kozak + hoiln(lambda phage) + ADH1 terminator

BBa_K809710
BBa_K809710 Version 1 (Component)
GAL promoter + kozak + ZIM17 + T7 RNAP + ADH1 terminator

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.