Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Han Zhu
Date created: 2007-02-26 12:00:00
Date modified: 2015-05-08 01:14:00
Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III
| Types | DnaRegion |
| Roles | Project engineered_region |
| Sequences | BBa_M31516_sequence (Version 1) |
Description
Contains refactored sequences of the M13 genome from HpaI [8669/8669] - BamHI [2220/2224].Notes
We wanted to refactor the genome of the M13 to faciliate future engineering. In doing so, we want to "clean up" the wildtype genes so that they are independent of each other and can be easily engineered independent of each other. We also want to delete unnecessary or obscure sequences for better human understanding of the M13 genome.Source
Sequence taken from the copy of the M13K07 genome available at: http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/m13ko7.txt| Sequence Annotation | Location | Component / Role(s) |
| promoter-g10 rbs-g10 promoter-g5 rbs-g5 g5 BsrGI rbs-g7 promoter-g8 rbs-g9 g8 promoter-g3 rbs-g3 g3 (incomplete) BamHI HpaI (incomplete) g2 (incomplete) g10 SnaBI | 381,428 480,495 786,835 827,842 843,1106 1021,1025 1092,1107 1155,1201 1190,1205 1301,1522 1500,1547 1563,1578 1579,2225 2220,2224 1,3 1,837 496,831 1268,1273 | promoter feature/promoter ribosome_entry_site feature/rbs feature/promoter promoter feature/rbs ribosome_entry_site CDS feature/protein sequence_feature feature/misc ribosome_entry_site feature/rbs feature/promoter promoter ribosome_entry_site feature/rbs CDS feature/protein feature/promoter promoter feature/rbs ribosome_entry_site CDS feature/protein sequence_feature feature/misc sequence_feature feature/misc feature/protein CDS feature/protein CDS feature/misc sequence_feature |