Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Amirah Khan
Date created: 2007-02-26 12:00:00
Date modified: 2015-05-08 01:14:00
Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III
| Types | DnaRegion |
| Roles | Project engineered_region |
| Sequences | BBa_M31513_sequence (Version 1) |
Description
This part has been refactored so that the re-engineering of genes within the part are made easier. We used the methods outlined by researches that refactored the T7 genome,which required inserting 'buffer regions' in between genes that were overlapping or embedded in each other.Notes
We had to consider the fact that basepairs within the regions that encoded for genes could not be altered too much since we want to maintain functionality of M13 after refactoring this segment of its genomeSource
The source for this part was the genmoic sequence of the M13 bacteriophage| Sequence Annotation | Location | Component / Role(s) |
| promoter-g10 rbs-g10 promoter g5 rbs-g5 g5 (ORF) BsrGI promoter-g8 rbs-g9 gene VIII promoter-g3 rbs-g3 gene III (ORF) incomplete BamHI gene IX (ORF) rbs-g8 BamHI Eag1 gene X (ORF) gene II (ORF) incomplete | 381,428 480,495 786,835 827,842 843,1106 1021,1025 1317,1364 1190,1205 1409,1630 1577,1624 1640,1655 1656,2302 2220,2224 1206,1306 1365,1381 1307,1311 1312,1317 496,831 1,831 | feature/promoter promoter ribosome_entry_site feature/rbs promoter feature/promoter feature/rbs ribosome_entry_site CDS feature/protein feature/misc sequence_feature feature/promoter promoter feature/rbs ribosome_entry_site feature/protein CDS promoter feature/promoter ribosome_entry_site feature/rbs CDS feature/protein sequence_feature feature/misc feature/protein CDS feature/rbs ribosome_entry_site feature/misc sequence_feature feature/misc sequence_feature CDS feature/protein CDS feature/protein |