BBa_K908005
BBa_K908005 Version 1 (Component)
Microcin B17, Gene E

BBa_K908006
BBa_K908006 Version 1 (Component)
Microcin B17, Gene F

BBa_K908008
BBa_K908008 Version 1 (Component)
Microcin B17, Gene G

BBa_K908009
BBa_K908009 Version 1 (Component)
Microcin C7, Gene A

BBa_K908010
BBa_K908010 Version 1 (Component)
Microcin C7, Gene B

BBa_K908011
BBa_K908011 Version 1 (Component)
Microcin C7, Gene C

BBa_K908012
BBa_K908012 Version 1 (Component)
Microcin C7, Gene D

BBa_K908013
BBa_K908013 Version 1 (Component)
Microcin C7, Gene E

BBa_K908014
BBa_K908014 Version 1 (Component)
Microcin C7, Gene F

BBa_K908015
BBa_K908015 Version 1 (Component)
Microcin B17, Gene A flanked by rbs and Attc sequence

BBa_K908016
BBa_K908016 Version 1 (Component)
Microcin B17, Gene B flanked by Attc sequence and rbs

BBa_K908017
BBa_K908017 Version 1 (Component)
Microcin B17, Gene C flanked by Attc sequence and rbs

BBa_K908018
BBa_K908018 Version 1 (Component)
Microcin B17, Gene F flanked by Attc sequence and rbs

BBa_K908019
BBa_K908019 Version 1 (Component)
Microcin C7, Gene E flanked by Attc sequence and rbs

BBa_K908020
BBa_K908020 Version 1 (Component)
Microcin C7, Gene C flanked by Attc sequence and rbs

pLac ColE2
BBa_K914001 Version 1 (Component)
pLac Colicin E2 Immunity protein

BBa_K908021
BBa_K908021 Version 1 (Component)
Microcin C7, Gene F flanked by Attc sequence and rbs

BBa_K914002
BBa_K914002 Version 1 (Component)
repressilator RBS Colicin E2 Immunity protein

BBa_J72218
BBa_J72218 Version 1 (Component)
kanamycin resistant p15A vector

BBa_K782016
BBa_K782016 Version 1 (Component)
10x[TALA+TALB] operator_CMV promoter_mCitrine

BBa_K782017
BBa_K782017 Version 1 (Component)
10x[TALA] operator_CMV promoter_mCitrine

BBa_K782018
BBa_K782018 Version 1 (Component)
10x[TALB] operator_CMV promoter_mCitrine

BBa_K782019
BBa_K782019 Version 1 (Component)
10x[TALA+TALB] operator_CMV promoter_fLuciferase

BBa_K782020
BBa_K782020 Version 1 (Component)
10x[TALA] operator_CMV promoter_fLuciferase

BBa_K782021
BBa_K782021 Version 1 (Component)
10x[TALB] operator_CMV promoter_fLuciferase

BBa_K782022
BBa_K782022 Version 1 (Component)
12x[TALD] operator_CMV promoter_fLuciferase

BBa_K782023
BBa_K782023 Version 1 (Component)
12x[NicTAL] operator_CMV promoter_fLuciferase

BBa_K782024
BBa_K782024 Version 1 (Component)
2x[NicTAL]+2x[TALD] operator_minimal promoter_mCitrine

BBa_K782025
BBa_K782025 Version 1 (Component)
4x[NicTAL]+4x[TALD] operator_minimal promoter_mCitrine

BBa_K782026
BBa_K782026 Version 1 (Component)
7x[TALA+TALB] operator_minimal promoter_mCitrine

BBa_K782027
BBa_K782027 Version 1 (Component)
7x[TALA] operator_minimal promoter_mCitrine

BBa_K782028
BBa_K782028 Version 1 (Component)
10x[TALA+TALB] operator_minimal promoter_mCitrine

BBa_K782029
BBa_K782029 Version 1 (Component)
10x[TALA] operator_minimal promoter_mCitrine

BBa_K769013
BBa_K769013 Version 1 (Component)
Promoter(Pbad)-RBS-luxA-DT

BBa_K769014
BBa_K769014 Version 1 (Component)
Promoter(Pbad)-RBS-luxD-DT

BBa_K769017
BBa_K769017 Version 1 (Component)
Promoter(Pbad)-RBS-luxA-DT-Promoter(high)-RBS-lux CDBFEG-DT

BBa_K769018
BBa_K769018 Version 1 (Component)
Promoter(Pbad)-RBS-luxD-DT-Promoter(high)-RBS-lux CABFEG-DT

BBa_K113021
BBa_K113021 Version 1 (Component)
Total construct: T7 polymerase + GFP/RFP + repressors + overlapping T7 promoter

Bxb1_Xis
BBa_K907001 Version 1 (Component)
Mycobacterium Phage Bxb1 gp47, DNA excisionase

BBa_K782030
BBa_K782030 Version 1 (Component)
10x[TALB] operator_minimal promoter_mCitrine

BBa_K782031
BBa_K782031 Version 1 (Component)
12x[TALD] operator_minimal promoter_mCitrine

BBa_K782032
BBa_K782032 Version 1 (Component)
10x[TALA+TALB] operator_minimal promoter_fLuciferase

BBa_K782033
BBa_K782033 Version 1 (Component)
10x[TALA] operator_minimal promoter_fLuciferase

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

Cello E. Coli Parts
Eco1C1G1T1_collection Version 1 (Collection)
These are the Cello parts for E. Coli circuits

BBa_I730002
BBa_I730002 Version 1 (Component)
pLacI-tetR repressor

BBa_K098997
BBa_K098997 Version 1 (Component)
cIts coding region

BBa_K098998
BBa_K098998 Version 1 (Component)
cIts coding region with rbs

BBa_S04136
BBa_S04136 Version 1 (Component)
terminator and cI promoter

BBa_S04137
BBa_S04137 Version 1 (Component)
heat sensitive cI QPI