BBa_K098995
BBa_K098995 Version 1 (Component)
heat sensitive cI QPI with high promoter

BBa_K098993
BBa_K098993 Version 1 (Component)
heat sensitive cI QPI with low promoter

BBa_K098991
BBa_K098991 Version 1 (Component)
cI promoter with GFP reporter

BBa_K105021
BBa_K105021 Version 1 (Component)
cI - operator

BBa_S04138
BBa_S04138 Version 1 (Component)
cI QPI with GFP reporter system

BBa_K098988
BBa_K098988 Version 1 (Component)
temperature sensitive cI inducible system with GFP reporter and high promoter

BBa_K098987
BBa_K098987 Version 1 (Component)
temperature sensitive cI inducible system with GFP reporter and low promoter

BBa_K098984
BBa_K098984 Version 1 (Component)
lac inducible mtrB (high expression of QPI)

BBa_K098982
BBa_K098982 Version 1 (Component)
lac inducible system with low promoter and GFP reporter

BBa_K098983
BBa_K098983 Version 1 (Component)
lac inducible mtrB (low expression of QPI)

BBa_K157010
BBa_K157010 Version 1 (Component)
glycine-serine linker fused to B-cell receptor transmembrane region; displays protein on cell surfac

Tm-Split-L
BBa_K157020 Version 1 (Component)
Tm-Split-Luciferase1/2

cI 434
BBa_R0052 Version 1 (Component)
Promoter (434 cI regulated)

BBa_K113007
BBa_K113007 Version 1 (Component)
pBad/araC->T7 polymerase

BBa_K113009
BBa_K113009 Version 1 (Component)
pBad/araC

BBa_K113019
BBa_K113019 Version 1 (Component)
pBad/araC->T7 polymerase->GFP

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

Tm-Splitlu
BBa_K157021 Version 1 (Component)
Tm-Splitluciferase2/2

linker
BBa_K157013 Version 1 (Component)
15 aa flexible glycine-serine protein domain linker; Freiburg standard

SP-AntiNIP
BBa_K157030 Version 1 (Component)
SP-AntiNIP-GGGSlinker-Tm-Splitluciferase2/2

SP-AntiNIP
BBa_K157031 Version 1 (Component)
SP-AntiNIP-GGGSlinker-Tm-Splitluciferase1/2

SP-Lipocal
BBa_K157038 Version 1 (Component)
SP-Lipocalin-GGGSlinker-Tm-Splitluciferase2/2

SP-Lipocal
BBa_K157039 Version 1 (Component)
SP-Lipocalin-GGGSlinker-Tm-Splitluciferase1/2

Q04510+RFP
BBa_K131018 Version 1 (Component)
Intermediate for the Response circuit

BBa_K131019
BBa_K131019 Version 1 (Component)
LuxO inducible RFP

BBa_K131026
BBa_K131026 Version 1 (Component)
AHL inducible GFP

BBa_K090501
BBa_K090501 Version 1 (Component)
Gram-Positive IPTG-Inducible Promoter

BBa_K090502
BBa_K090502 Version 1 (Component)
Gram-Positive Xylose-Inducible Promoter

BBa_K123002
BBa_K123002 Version 1 (Component)
LacIQ ERE TetR

BBa_K082002
BBa_K082002 Version 1 (Component)
RBS defines RBS efficiency

BBa_K082004
BBa_K082004 Version 1 (Component)
LacI- wild type

BBa_K082005
BBa_K082005 Version 1 (Component)
LacI-Mutant

BBa_K090505
BBa_K090505 Version 1 (Component)
''Bacillus subtilis'' consensus RBS

BBa_K090506
BBa_K090506 Version 1 (Component)
''Bacillus subtilis'' weak RBS

BBa_K082012
BBa_K082012 Version 1 (Component)
B0034-LacI

BBa_K082034
BBa_K082034 Version 1 (Component)
lacI regulated GFP generator

BBa_K156011
BBa_K156011 Version 1 (Component)
aadA (streptomycin 3'-adenyltransferase)

BBa_K082028
BBa_K082028 Version 1 (Component)
R0040-B0034-LacI

RBS-cI
BBa_K093006 Version 1 (Component)
cI repressor with RBS

RBS-cI 434
BBa_K093007 Version 1 (Component)
C0052 + RBS

BBa_K082026
BBa_K082026 Version 1 (Component)
B0034-LacIM1-B0015

BBa_K082025
BBa_K082025 Version 1 (Component)
B0034-LacI-B0015

BBa_K082016
BBa_K082016 Version 1 (Component)
LacIM1-B0015

Regional
BBa_K106676 Version 1 (Component)
Regional silencing construct

BBa_K142008
BBa_K142008 Version 1 (Component)
lacI IS mutant R197A with RBS and terminator

BBa_K142009
BBa_K142009 Version 1 (Component)
lacI IS mutant R197F with RBS and terminator

BBa_K142010
BBa_K142010 Version 1 (Component)
lacI IS mutant T276A with RBS and terminator

BBa_K142011
BBa_K142011 Version 1 (Component)
lacI IS mutant T276Fwith RBS and terminator

BBa_K142012
BBa_K142012 Version 1 (Component)
lacI IS mutant R197A T276A with RBS and terminator