BBa_K091230BBa_K091230 Version 1 (Component)cI promoter+RBS+RFP+TT
BBa_I744111BBa_I744111 Version 1 (Component)Tc-driven cI (lambda) generator
BBa_K1647000BBa_K1647000 Version 1 (Component)CI operator site with GFP
BBa_J13101BBa_J13101 Version 1 (Component)LacI repressed lambda CI generator
PL GFPBBa_K193000 Version 1 (Component)GFP reporter regulated by CI.
BBa_K1053300BBa_K1053300 Version 1 (Component)PLtetO-1-RBS-cI-DT
BBa_K909004BBa_K909004 Version 1 (Component)cI with strong ribosomal binding site
BBa_I719022BBa_I719022 Version 1 (Component)cI promoter with GFP reporter
BBa_K1159310BBa_K1159310 Version 1 (Component)34 AA flexible linker with C-terminal TEV cleavage site (GGGGS)x5-TEV-site-linker) in RFC[25]
BBa_K648032BBa_K648032 Version 1 (Component)Slightly weaker RBS with cI repressor
BBa_K648033BBa_K648033 Version 1 (Component)Very weak RBS with cI repressor
BBa_K318509BBa_K318509 Version 1 (Component)lacI pL + RBS + cI LVA + TT
Plamda-GFPBBa_I763011 Version 1 (Component)promoter lambda (cI regulated) with GFP (+LVA) reporter
BBa_K1707004BBa_K1707004 Version 1 (Component)TetR-ssRA repressor with the promoter of cI
BBa_K415011BBa_K415011 Version 1 (Component)PtetR : RBS : LuxR : Term : PluxR/cI-OR : RBS : mCherry : Term : Plux/cI-OR : RBS : LuxI
BBa_K077039BBa_K077039 Version 1 (Component)cI under control of the plac promotor
BBa_K176017BBa_K176017 Version 1 (Component)pCI(R0051)(lambda CI-)->RBS+GFP+T
BBa_K145105BBa_K145105 Version 1 (Component)cI under T7 and P<sub>R</sub> dual promotor
BBa_K077019BBa_K077019 Version 1 (Component)pluxR with cI (lambda) behind a normal RBS
BBa_I3401BBa_I3401 Version 1 (Component)Lambda cI switch input device (B0034.C0051.B0015)
BBa_K584011BBa_K584011 Version 1 (Component)Lac-Lux hybrid promotor + CrtEBI + CI repressor + INP
BBa_K611017BBa_K611017 Version 1 (Component)cI Lambda Repressor and Promoter Wild Type Control
PLac-LacY-BBa_I763013 Version 1 (Component)LacY and cI coding device switched on by IPTG
BBa_I758601BBa_I758601 Version 1 (Component)Screen for binding affinity of mutant cI lambda to promotor sites
BBa_I758600BBa_I758600 Version 1 (Component)Screen for binding affinity of mutant cI lambda to promotor sites
BBa_K145112BBa_K145112 Version 1 (Component)cI under T7 and PR<sub>R</sub> dual promotor
BBa_K177035BBa_K177035 Version 1 (Component)cI repressor from E. coli phage lambda (+LVA) under control of RBS.3 (medium)
BBa_K584008BBa_K584008 Version 1 (Component)Lambda cI and LuxR regulated hybrid promotor + RBS + MelA + RBS + AFP + term
BBa_K415005BBa_K415005 Version 1 (Component)pLux/cI-OR : RBS-mCherry : Term : p(tetR) : RBS-luxR : Term
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.