BBa_J100272
BBa_J100272 Version 1 (Component)
rClone Red Version 2: Device for GGA Cloning and Testing RBS elements and Riboswitches

BBa_J100282
BBa_J100282 Version 1 (Component)
rClone Red Version 2 with RBS: Device for GGA Cloning and Testing RBS elements and Riboswitches

BBa_J100297
BBa_J100297 Version 1 (Component)
rClone Red Version 1.5 with RBS 2.0: Device for GGA Cloning and Testing RBS elements and Riboswitche

BBa_J100296
BBa_J100296 Version 1 (Component)
rClone Red Version 2 with RBS 2.0: Device for GGA Cloning and Testing RBS elements and Riboswitches

Suppor Dev
BBa_K1433013 Version 1 (Component)
rT-rRFP-rRBS-attB-P-attP-RBS-lambda red-RBS-GFP-T-P-RBS-gp47-tag-T

BBa_K1159120
BBa_K1159120 Version 1 (Component)
Red light triggered TEV Protease with FRET Reporter for plants translation unit (PhyB/PIF6 version)

BBa_K325239
BBa_K325239 Version 1 (Component)
Red Firefly Luciferase and LRE (under pBAD)<BR><i>L. Cruciata<BR>(E. coli optimised)</i>

BBa_K1159119
BBa_K1159119 Version 1 (Component)
Red light triggered TEV Protease with FRET Reporter for plants translation unit (PhyB/PIF3 version)

SUPPORT DE
BBa_K1433022 Version 1 (Component)
rT-rKan-rRBS-attB-P-attP-RBS-lambda red-RBS-Chl-T-P-RBS-gp47-tag-T

7x His
BBa_T2001 Version 1 (Component)
7x His tag head domain

BBa_K360115
BBa_K360115 Version 1 (Component)
Mutated red luciferase based on BBa_I712019

BBa_J70622
BBa_J70622 Version 1 (Component)
RFC12 cMyc Tag Head Domain

BBa_J70624
BBa_J70624 Version 1 (Component)
RFC12 FLAG Tag Head Domain

BBa_K892000
BBa_K892000 Version 1 (Component)
Red light responsive luciferase

BBa_J119395
BBa_J119395 Version 1 (Component)
tClone Red containing the RS3 Riboswitch

BBa_I20292
BBa_I20292 Version 1 (Component)
There is no limit to what a man can do or where he can go if...

BBa_K892999
BBa_K892999 Version 1 (Component)
red light responsive inverter driving luciferase

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.