BBa_I766679
BBa_I766679 Version 1 (Component)
YopJ-RR under medium constitutive promotor

BBa_I766675
BBa_I766675 Version 1 (Component)
YopH-RR under medium constitutive promoter

BBa_K1369006
BBa_K1369006 Version 1 (Component)
pBAD FsrA, pFsrA-GFP, RR with no recruitment

BBa_K1369009
BBa_K1369009 Version 1 (Component)
pBAD LamA, pLamA-GFP, RR with no recruitment

BBa_K1632030
BBa_K1632030 Version 1 (Component)
fim switch[default ON](Tokyo_Tech/J23119)_rbs_gfp_rbs_rhlI

BBa_K1632032
BBa_K1632032 Version 1 (Component)
fim switch[default ON](Tokyo_Tech/J23119)_rbs_gfp_rbs_lasI

BBa_K1632031
BBa_K1632031 Version 1 (Component)
fim switch[default OFF](Tokyo_Tech/J23119)_rbs_gfp_rbs_rhlI

BBa_K1632033
BBa_K1632033 Version 1 (Component)
fim switch[default OFF](Tokyo_Tech/J23119)_rbs_gfp_rbs_lasI

BBa_K1077007
BBa_K1077007 Version 1 (Component)
J23100 fim switch b0034 amilCP ON orientation

BBa_K758003
BBa_K758003 Version 1 (Component)
UAS, this part consists of five UAS sequences and hsp70 TATA.

BBa_I766019
BBa_I766019 Version 1 (Component)
Ste2-GFP-RR under endogenous promoter

CBDcex(T7)
BBa_K863102 Version 1 (Component)
Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)

BBa_K377704
BBa_K377704 Version 1 (Component)
FimB

IR-3-GFP
BBa_K658017 Version 1 (Component)
LuxI->LuxR->lux pR-3->GFP

BBa_K1369007
BBa_K1369007 Version 1 (Component)
pBAD FsrA-SH3pep, pFsrA-GFP, RR with direct recruitment (SH3 peptide). Ideal pBad concentrations wil

BBa_I729009
BBa_I729009 Version 1 (Component)
Time device

BBa_K1369010
BBa_K1369010 Version 1 (Component)
pBAD LamA-L-SH3pep, pLamA-GFP, RR with direct recruitment (SH3 peptide). Ideal pBad concentrations w

BBa_K2052016
BBa_K2052016 Version 1 (Component)
FimH site directed mutated with RPMrel and ButCoat

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.