BBa_K102908
BBa_K102908 Version 1 (Component)
TA10 gate from synthetic algorithm v1.1

BBa_K102909
BBa_K102909 Version 1 (Component)
TA11 gate from synthetic algorithm v1.1

BBa_K102910
BBa_K102910 Version 1 (Component)
TA12 gate from synthetic algorithm v1.1

gpS
BBa_K836006 Version 1 (Component)
Lysis protein S from Enterobacteria phage lambda (codon usage optimized for R. opacus)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K157010
BBa_K157010 Version 1 (Component)
glycine-serine linker fused to B-cell receptor transmembrane region; displays protein on cell surfac

BBa_K131026
BBa_K131026 Version 1 (Component)
AHL inducible GFP

BBa_K102911
BBa_K102911 Version 1 (Component)
TA13 gate from synthetic algorithm v1.2

p_qrr4
BBa_K131017 Version 1 (Component)
p_qrr4 from <I>Vibrio harveyi</I>

linker
BBa_K157013 Version 1 (Component)
15 aa flexible glycine-serine protein domain linker; Freiburg standard

BBa_K143075
BBa_K143075 Version 1 (Component)
B. subtilis AmyE locus GFP output promoter and RBS tester

BBa_K143077
BBa_K143077 Version 1 (Component)
P43-gsiB GFP expression construct

BBa_K143078
BBa_K143078 Version 1 (Component)
P43-spoVG GFP expression construct

BBa_K143079
BBa_K143079 Version 1 (Component)
Pveg-spoVG GFP expression construct

BBa_K125810
BBa_K125810 Version 1 (Component)
slr2016 signal sequence + GFP fusion for secretion of GFP

BBa_K082003
BBa_K082003 Version 1 (Component)
GFP(+LVA)

BBa_K082037
BBa_K082037 Version 1 (Component)
RhlI & GFP generator

BBa_K082034
BBa_K082034 Version 1 (Component)
lacI regulated GFP generator

BBa_K102905
BBa_K102905 Version 1 (Component)
TA7 gate from synthetic algorithm v1.1

BBa_K156009
BBa_K156009 Version 1 (Component)
OFP (orange fluorescent protein)

SBFP2
BBa_K156010 Version 1 (Component)
SBFP2 (strongly enhanced blue fluorescent protein)

BBa_K082024
BBa_K082024 Version 1 (Component)
B0034-GFP(LVA)-B0015

BBa_K082019
BBa_K082019 Version 1 (Component)
GFP generator

BBa_K082015
BBa_K082015 Version 1 (Component)
GFP(LVA)-B0015

BBa_K106671
BBa_K106671 Version 1 (Component)
GFP, Aar1 AD part

BBa_K106674
BBa_K106674 Version 1 (Component)
GFP under medium constitutive promoter, 5

BBa_K106679
BBa_K106679 Version 1 (Component)
GFP, under pheromone-inducible promoter, 5' LexA Ops

BBa_K116510
BBa_K116510 Version 1 (Component)
GFP under control of pOmpf promoter(K116500+E0240)

BBa_S04147
BBa_S04147 Version 1 (Component)
The Partial Butanol Operon (5 inserts from I725021 to I725025)

BBa_I0466
BBa_I0466 Version 1 (Component)
RhlR Protein Generator

BBa_K136061
BBa_K136061 Version 1 (Component)
fliL promoter + WT RBS ( K136009) - GFP

BBa_K116503
BBa_K116503 Version 1 (Component)
GFP under control of pOmpC promoter.R0082+E0240

BBa_K136062
BBa_K136062 Version 1 (Component)
fliL promoter + WT RBS ( K136009) - GFP

BBa_K136065
BBa_K136065 Version 1 (Component)
flhB promoter + WT RBS - GFP

BBa_K116520
BBa_K116520 Version 1 (Component)
GFP under control of RpaA activated pOmpF promoter

BBa_K116523
BBa_K116523 Version 1 (Component)
GFP under control of RpaA activated pOmpC promoter

BBa_J04430
BBa_J04430 Version 1 (Component)
GFP coding device switched on by IPTG

BBa_K142073
BBa_K142073 Version 1 (Component)
lac-controlled GFP generator with constitutive lacI expression cassette

BBa_K142074
BBa_K142074 Version 1 (Component)
lac-controlled GFP-LVA generator with constitutive lacI expression cassette

BBa_K133086
BBa_K133086 Version 1 (Component)
CMV-SS-gyrEC-linker-(TMTIR)TLR9-GFP-Histop

BBa_K133087
BBa_K133087 Version 1 (Component)
CMV-SS-gyrHP-linker-(TMTIR)TLR9-GFP-Histop

BBa_K133113
BBa_K133113 Version 1 (Component)
CMV-SS-HA-multiHP-(TMTIR)TLR4-GFP-Histop

pLac+GFP
BBa_I763004 Version 1 (Component)
GFP coding device switched on by IPTG

RBS.TetR
BBa_I15040 Version 1 (Component)
RBS.TetR from pASKIBA3

BBa_K116000
BBa_K116000 Version 1 (Component)
A homolog of OCT3 (organic cation transporter 3) from E coli. K12

BBa_K116601
BBa_K116601 Version 1 (Component)
HtlB (ftsH) coding region from E. coli

BBa_K116001
BBa_K116001 Version 1 (Component)
nhaA promoter, that can be regulated by pH and nhaR protein.