BBa_J06905
BBa_J06905 Version 1 (Component)
Lambda cI857 (Lambda II) QPI regulated by 434 cI with mCherry

BBa_J06915
BBa_J06915 Version 1 (Component)
Lambda cI857 (Lambda II) QPI regulated by 434 cI with EYFP

BBa_M302009
BBa_M302009 Version 1 (Component)
Region between HpaI site in gene II and BamHI site in gene III

BBa_J06965
BBa_J06965 Version 1 (Component)
434 cI/lambda cI857 (Lambda II) RS flip-flop with EYFP

BBa_K1159005
BBa_K1159005 Version 1 (Component)
Secretory Erythromycin Esterase Type II (IgKappa-SigP_EreB) in RFC[25] N-Part

BBa_K1351036
BBa_K1351036 Version 1 (Component)
Quorum sensing two component system (AIP-II sensing histidine kinase agrC, response regulator agrA)

BBa_K223050
BBa_K223050 Version 1 (Component)
SoxR - SoxS System with Blh and RALDH II system and Lac promoter

BBa_J06574
BBa_J06574 Version 1 (Component)
Construction intermediate: cI857 (Lamda II) with RBS and forward terminator (B0034.J06503.B0014)

BBa_M31517
BBa_M31517 Version 1 (Component)
Refactored M13KO7 genome from HpaI site in gene II through BamHI site in gene III

BBa_K1022126
BBa_K1022126 Version 1 (Component)
pT7: RBS: His6- SUMO: Magainin II: RBS: TetR:TT : pTet: cI: TT : pcI: Ulp : Lysis

BBa_K1159309
BBa_K1159309 Version 1 (Component)
19 AA Linker with N-terminal <i>Strep</i>-tag II and C-terminal TEV cleavage site in RFC[25]

BBa_K887003
BBa_K887003 Version 1 (Component)
Plac+B0034+alsS+zif268+B0034+PBS II+ilvC+B0034+HIVC+ilvD+Ptet+B0032+kivD+B0015

luxR-ndh
BBa_K1036000 Version 1 (Component)
lux pL controlled luxR with lux pR controlled ndh (LVA-tag) coding for NADH dehydrogenase II

BBa_M31513
BBa_M31513 Version 1 (Component)
Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III

BBa_M31516
BBa_M31516 Version 1 (Component)
Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III

Strep-Tag
BBa_I757014 Version 1 (Component)
Strep-Tag II affinity tag

BBa_M31114
BBa_M31114 Version 1 (Component)
M13, gene II from HpaI to the end [1-831bp]

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.