pSBBs2EBBa_K823027 Version 1 (Component)pSB<sub>Bs</sub>2E: Empty backbone for integration into Bacillus subtilis lacA locus
BBa_J119104BBa_J119104 Version 1 (Component)pT7-RBS-GFP-pLac+tRNA CCACC+pLac+tRNA AGGAC+pLac+tRNA CUACU
BBa_J119107BBa_J119107 Version 1 (Component)pT7-RBS-GFP-pLac+tRNA CCACC+pLac+tRNA AGGAC+pLac+tRNA CUACC
BBa_K1974033BBa_K1974033 Version 1 (Component)T7 Promoter+RBS+Hv1a+GS linker+snowdrop-lectin+linker+6X His-Tag
BBa_K1053209BBa_K1053209 Version 1 (Component)Pconst.- RBS-YF1/FixJ-DT- Pfixk2- taRNA- DT- Pconst.- HHR*- GFP- DT
BBa_I758601BBa_I758601 Version 1 (Component)Screen for binding affinity of mutant cI lambda to promotor sites
BBa_I758600BBa_I758600 Version 1 (Component)Screen for binding affinity of mutant cI lambda to promotor sites
BBa_K145112BBa_K145112 Version 1 (Component)cI under T7 and PR<sub>R</sub> dual promotor
BBa_R4030BBa_R4030 Version 1 (Component)PoPS/RiPS Generator composed of the Tet promoter and a strong RBS (R0040.E0030)
pSBBs4SBBa_K823022 Version 1 (Component)pSB<sub>Bs</sub>4S: Empty backbone for integration into <i/>Bacillus subtilis thrC</i> locus
pSBBs1CBBa_K823023 Version 1 (Component)pSB<sub>Bs</sub>1C: Empty backbone for integration into <i>Bacillus subtilis</i> <i>amyE</i> locus
BBa_J329040BBa_J329040 Version 1 (Component)luxIR QS System with B0034 RBSs and LVA-tagged GFP
BBa_K165100BBa_K165100 Version 1 (Component)Gli1 bs + LexA bs + mCYC + LexA repressor (mCherryx2 tagged) on pRS304*
BBa_K165101BBa_K165101 Version 1 (Component)Zif268-HIV bs + LexA bs + mCYC + Zif268-HIV repressor (mCherryx2 tagged) on pRS304*
BBa_K180005BBa_K180005 Version 1 (Component)GoL - Primary plasmid (part 1)/RPS - Paper primary plasmid (part 1) [LuxR generator]
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.