BBa_J04431
BBa_J04431 Version 1 (Component)
GFP Coding Device with promoter, RBS, GFP with LVA tag, and Terminator

BBa_K1361007
BBa_K1361007 Version 1 (Component)
Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag at a relative

BBa_K2082252
BBa_K2082252 Version 1 (Component)
RFP under the control of an optimized lacZ promoter with lambda cI binding site combined with SH2:cI

BBa_K1179005
BBa_K1179005 Version 1 (Component)
(Acyl-TyA-rtTA3-His) A membrane/exosome targeting rtTA3 fusion to send through exosomes with His tag

MLS
BBa_K1119001 Version 1 (Component)
Mitochondrial Leader Sequence with RFC25 standard

BBa_K2123117
BBa_K2123117 Version 1 (Component)
Novel RFP device regulated by mercury: MerR (regulatory protein) + Stationary phase with mer operato

BBa_K1463807
BBa_K1463807 Version 1 (Component)
RFP under J23112 promoter

BBa_K1059016
BBa_K1059016 Version 1 (Component)
GFP-LVA under J23101 control

BBa_K936012
BBa_K936012 Version 1 (Component)
Leader sequence that brings protein to periplasm

Adapter Bi
BBa_K1807015 Version 1 (Component)
This device allows for the IPTG-inducible expression of lacZα peptide which in the presence of

BBa_K1463771
BBa_K1463771 Version 1 (Component)
MotA and MotB under J23112 promoter

BBa_K639004
BBa_K639004 Version 1 (Component)
rrnB P1-LacI-pLac-mCherry plausible stress sensor

BBa_K1323019
BBa_K1323019 Version 1 (Component)
Hfq expression cassette under a xylose inducible promoter

BBa_K112228
BBa_K112228 Version 1 (Component)
{pelB>} The pelB leader without stop codon, BBb format

BBa_I766223
BBa_I766223 Version 1 (Component)
HMTM1-EE under weak constitutive promoter

BBa_K1463753
BBa_K1463753 Version 1 (Component)
MotB and B0032 RBS under J23103 promoter

BBa_K1463703
BBa_K1463703 Version 1 (Component)
MotA and B0032 RBS under J23103 promoter

BBa_K1541025
BBa_K1541025 Version 1 (Component)
sfGFP under promoter P(Lux) with riboregulator RR12y

BBa_K077041
BBa_K077041 Version 1 (Component)
AiiA and cII under control of plac promotor

BBa_K145112
BBa_K145112 Version 1 (Component)
cI under T7 and PR_R dual promotor

BBa_J22121
BBa_J22121 Version 1 (Component)
Lac Y gene under the rec A(SOS) promoter in plasmid pSB2K3

BBa_K323164
BBa_K323164 Version 1 (Component)
VioA and VioB enzymes fused with zinc fingers under pBAD promoter in pSB4K5

BBa_K323163
BBa_K323163 Version 1 (Component)
VioC, VioD and VioE enzymes fused with zinc fingers under pBAD promoter in pSB4C5

iGEM Parts Registry
igem_collection Version 1 (Collection)
The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.