BBa_K091230BBa_K091230 Version 1 (Component)cI promoter+RBS+RFP+TT
BBa_I744111BBa_I744111 Version 1 (Component)Tc-driven cI (lambda) generator
BBa_I10200BBa_I10200 Version 1 (Component)434 cI repressor protein generator
BBa_K1647000BBa_K1647000 Version 1 (Component)CI operator site with GFP
BBa_J13101BBa_J13101 Version 1 (Component)LacI repressed lambda CI generator
PL GFPBBa_K193000 Version 1 (Component)GFP reporter regulated by CI.
BBa_K1053300BBa_K1053300 Version 1 (Component)PLtetO-1-RBS-cI-DT
BBa_K909004BBa_K909004 Version 1 (Component)cI with strong ribosomal binding site
BBa_M11402BBa_M11402 Version 1 (Component)5' UTR and RBS of psbA2 gene in Synechocystis sp. PCC 6803
BBa_I719022BBa_I719022 Version 1 (Component)cI promoter with GFP reporter
BBa_K648032BBa_K648032 Version 1 (Component)Slightly weaker RBS with cI repressor
BBa_K648033BBa_K648033 Version 1 (Component)Very weak RBS with cI repressor
BBa_K318509BBa_K318509 Version 1 (Component)lacI pL + RBS + cI LVA + TT
Plamda-GFPBBa_I763011 Version 1 (Component)promoter lambda (cI regulated) with GFP (+LVA) reporter
BBa_K1707004BBa_K1707004 Version 1 (Component)TetR-ssRA repressor with the promoter of cI
BBa_K188222BBa_K188222 Version 1 (Component)RBS+cI repressor from phage 434 (+LVA)
BBa_K415011BBa_K415011 Version 1 (Component)PtetR : RBS : LuxR : Term : PluxR/cI-OR : RBS : mCherry : Term : Plux/cI-OR : RBS : LuxI
BBa_K077039BBa_K077039 Version 1 (Component)cI under control of the plac promotor
BBa_J06570BBa_J06570 Version 1 (Component)Construction intermediate: 434 cI with RBS (B0034.C0052)
BBa_K145105BBa_K145105 Version 1 (Component)cI under T7 and P<sub>R</sub> dual promotor
BBa_K077019BBa_K077019 Version 1 (Component)pluxR with cI (lambda) behind a normal RBS
BBa_I3401BBa_I3401 Version 1 (Component)Lambda cI switch input device (B0034.C0051.B0015)
BBa_K584011BBa_K584011 Version 1 (Component)Lac-Lux hybrid promotor + CrtEBI + CI repressor + INP
BBa_K611017BBa_K611017 Version 1 (Component)cI Lambda Repressor and Promoter Wild Type Control
PLac-LacY-BBa_I763013 Version 1 (Component)LacY and cI coding device switched on by IPTG
BBa_K077016BBa_K077016 Version 1 (Component)cI (lambda) promoter with normal RBS, AiiA and terminators
BBa_I758601BBa_I758601 Version 1 (Component)Screen for binding affinity of mutant cI lambda to promotor sites
BBa_I758600BBa_I758600 Version 1 (Component)Screen for binding affinity of mutant cI lambda to promotor sites
BBa_K145112BBa_K145112 Version 1 (Component)cI under T7 and PR<sub>R</sub> dual promotor
BBa_K177035BBa_K177035 Version 1 (Component)cI repressor from E. coli phage lambda (+LVA) under control of RBS.3 (medium)
BBa_K584008BBa_K584008 Version 1 (Component)Lambda cI and LuxR regulated hybrid promotor + RBS + MelA + RBS + AFP + term
BBa_K415005BBa_K415005 Version 1 (Component)pLux/cI-OR : RBS-mCherry : Term : p(tetR) : RBS-luxR : Term
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.