BBa_K660001
BBa_K660001 Version 1 (Component)
LOV2 Domain with 6xHIS tag

BBa_K1230002
BBa_K1230002 Version 1 (Component)
YFP generator with LsrA promoter, with terminator as a prefix

BBa_K1499205
BBa_K1499205 Version 1 (Component)
MntH with amber stop codon with promoter for expression, no tRNA

BBa_J70597
BBa_J70597 Version 1 (Component)
J70577 cut with BsaXI and with 6his inserts (J70564-fi) added

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1051705
BBa_K1051705 Version 1 (Component)
MER1 with a point synonymous mutation

Yeb-Art175
BBa_K1659003 Version 1 (Component)
Artilysin Art-175 fused at N-terminal with porin-dependent YebF secretion signal protein

BBa_K648125
BBa_K648125 Version 1 (Component)
Or tester with cI repressor with slightly weak RBS

BBa_K648126
BBa_K648126 Version 1 (Component)
Or tester with cI repressor with very weak RBS

BBa_K359007
BBa_K359007 Version 1 (Component)
AIP sensor with FepA pore

BBa_K1001750
BBa_K1001750 Version 1 (Component)
AraC sensor with CFP reporter

BBa_K216016
BBa_K216016 Version 1 (Component)
PyeaR with bacterial luciferase

BBa_J58117
BBa_J58117 Version 1 (Component)
Mutated OmpR promoter with inverter TetR

BBa_I14002
BBa_I14002 Version 1 (Component)
LacI with strong RBS

BBa_I14006
BBa_I14006 Version 1 (Component)
CinR with strong RBS

BBa_I14003
BBa_I14003 Version 1 (Component)
TetR with strong RBS

BBa_I14010
BBa_I14010 Version 1 (Component)
araC with strong RBS

BBa_M36521
BBa_M36521 Version 1 (Component)
strong 5' bicistronic UTR, with ATG at end

BBa_K1162012
BBa_K1162012 Version 1 (Component)
GFP with 10x His Tag

BBa_K654061
BBa_K654061 Version 1 (Component)
Wax Ester Synthase with 8-His Tag

BBa_K654063
BBa_K654063 Version 1 (Component)
Fatty Aldehyde Decarbonylase with 8-His Tag

BBa_K264046
BBa_K264046 Version 1 (Component)
UP Element Design 5 with BBa_J23101

BBa_K264047
BBa_K264047 Version 1 (Component)
UP Element Design 6 with BBa_J23101

BBa_K264048
BBa_K264048 Version 1 (Component)
UP Element Design 7 with BBa_J23101

BBa_K264045
BBa_K264045 Version 1 (Component)
UP Element Design 4 with BBa_J23101

BBa_J70605
BBa_J70605 Version 1 (Component)
J70590 cut with BsaXI and with GSGSGS inserts (J70556-fi,ri) added

BBa_K993003
BBa_K993003 Version 1 (Component)
Urease generator with ROSE thermometer

BBa_K1463751
BBa_K1463751 Version 1 (Component)
MotB with B0032 RBS

BBa_P100109
BBa_P100109 Version 1 (Component)
LMS ORF with RBS

BBa_K122016
BBa_K122016 Version 1 (Component)
4CL:STS with CYC1 terminator.

BBa_K660616
BBa_K660616 Version 1 (Component)
pBAD with strong RBS

BBa_K1412924
BBa_K1412924 Version 1 (Component)
GFP generator with J23101

BBa_K2005052
BBa_K2005052 Version 1 (Component)
mCherry with T7 expression

BBa_J69666
BBa_J69666 Version 1 (Component)
Potentially J69516 with J69511

RBS + ButC
BBa_K2052015 Version 1 (Component)
ButCoat with strong RBS

BBa_J09340
BBa_J09340 Version 1 (Component)
lacI with medium RBS

BBa_P100101
BBa_P100101 Version 1 (Component)
SAMT ORF with RBS

BBa_K675004
BBa_K675004 Version 1 (Component)
Arsenic Detector With Wintergreen

BBa_P100113
BBa_P100113 Version 1 (Component)
PAD1 ORF with RBS

BBa_K2117002
BBa_K2117002 Version 1 (Component)
TEF1 promoter with proinsulin

BBa_K301001
BBa_K301001 Version 1 (Component)
pTAC with RFP reporter

BBa_P100111
BBa_P100111 Version 1 (Component)
Oe23 ORF with RBS

BBa_P100105
BBa_P100105 Version 1 (Component)
SAAT ORF with RBS

BBa_K1412999
BBa_K1412999 Version 1 (Component)
GFP generator with J23115

BBa_K216010
BBa_K216010 Version 1 (Component)
ompC promoter with lacZ

BBa_K675005
BBa_K675005 Version 1 (Component)
Arsenic Detector With GFP

BBa_P100110
BBa_P100110 Version 1 (Component)
LIS ORF with RBS

BBa_K818310
BBa_K818310 Version 1 (Component)
AmilGFP with alsT regulator

BBa_I2057
BBa_I2057 Version 1 (Component)
Promoter Tester with RFP

BBa_K1960900
BBa_K1960900 Version 1 (Component)
P2 promoter with RBS