BBa_J44006BBa_J44006 Version 1 (Component)pLac-RBS-HinLVA-TT-HixC-pBAD-HixC-RBS-TetF-TT-RE
BBa_J44007BBa_J44007 Version 1 (Component)pLac-RBS-Hin-TT-HixC-pBADrev-HixC-RBS-RE-TT-TetF
BBa_J44005BBa_J44005 Version 1 (Component)pLac-RBS-Hin-TT-HixC-pBAD-HixC-RBS-TetF-TT-RE
BBa_K133039BBa_K133039 Version 1 (Component)T7-CF-UREB-RGD-Histop
med-ABO_2830 Version 1 (Component) BBa_I715069BBa_I715069 Version 1 (Component)Test part to see if RE and pLac +hixc can be expressed with GFP
BBa_K133037BBa_K133037 Version 1 (Component)T7-CF213-UB33-CF215-RGD-Histop
BBa_S03641BBa_S03641 Version 1 (Component)HixC : TetB-RBS<sub>rev</sub>-HixC
BBa_K133020BBa_K133020 Version 1 (Component)CMV-CF213-multiHP-CF215-RGD-Histop (term.)
BBa_S03638BBa_S03638 Version 1 (Component)HixC-pBad<sub>rev</sub>-HixC : RBS-TetF
BBa_M11085BBa_M11085 Version 1 (Component)E coli outer membrane protein C (ompC) with BamHI RE site for insertion of gene to be expressed on o
BBa_K133023BBa_K133023 Version 1 (Component)CMV-ss-CF213-multiHP-CF215-RGD-Histop (term)
BBa_S03674BBa_S03674 Version 1 (Component)(-1,2) HixC-pBad<sub>rev</sub>-HixC : RBS-TetF-HixC
BBa_J22121BBa_J22121 Version 1 (Component)Lac Y gene under the rec A(SOS) promoter in plasmid pSB2K3
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.