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BBa_J44007

BBa_J44007 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J44007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Brad Ogden

Date created: 2006-08-21 11:00:00

Date modified: 2015-08-31 04:08:49

pLac-RBS-Hin-TT-HixC-pBADrev-HixC-RBS-RE-TT-TetF

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Details

Types	DnaRegion
Roles	engineered_region Device
Sequences	BBa_J44007_sequence (Version 1)

Description

This a device that uses Hin to produce Hin recombinase that can flip the promotor pBADrev for Tet resistance; this device can turn on/off the genes for Tet resistance.

Notes

Possibility of read-thru of the pBAD during transcription.

Source

(BBa_B0030), (BBa_B0015), (BBa_R0010), (BBa_J31007), (BBa_J3101)- from registry. (BBa_J31000) - from Davidson College. (BBa_J44000) - made in collaboration with Missouri Western/Davidson.
(BBa_J44002) - from Missouri Western State University

Components

Access	Instance	Definition
public public public public public public public public public public public public public	BBa_R0010 BBa_B0030 BBa_J31000 BBa_B0010 BBa_B0012 BBa_J44000 BBa_J44002 BBa_J44000 BBa_B0030 BBa_J31007 BBa_B0010 BBa_B0012 BBa_J3101	LacI BBa_B0030 Hin BBa_B0010 BBa_B0012 hixC BBa_J44002 hixC BBa_B0030 tetA(C)f BBa_B0010 BBa_B0012 RE

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010 BBa_B0030 BBa_J31000 BBa_B0010 BBa_B0012 BBa_J44000 BBa_J44002 BBa_J44000 BBa_B0030 BBa_J31007 BBa_B0010 BBa_B0012 BBa_J3101	1,200 209,223 230,802 811,890 899,939 948,973 982,1111 1120,1145 1154,1168 1175,2365 2374,2453 2462,2502 2511,2587	LacI BBa_B0030 Hin BBa_B0010 BBa_B0012 hixC BBa_J44002 hixC BBa_B0030 tetA(C)f BBa_B0010 BBa_B0012 RE

Other Properties

igem#experience	Issues
igem#sampleStatus	Not in stock
igem#status	Unavailable
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_J44007/1