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BBa_J44006

BBa_J44006 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J44006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Brad Ogden

Date created: 2006-08-21 11:00:00

Date modified: 2015-08-31 04:08:49

pLac-RBS-HinLVA-TT-HixC-pBAD-HixC-RBS-TetF-TT-RE

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Details

Types	DnaRegion
Roles	Device engineered_region
Sequences	BBa_J44006_sequence (Version 1)

Description

This a device that uses Hin to produce Hin recombinase that can flip the promotor pBAD for Tet resistance; this device can turn on/off the genes for Tet resistance. It uses HinLVA as a degradation tag, which will terminate the flipping of the HixC when the HinLVA is completely degraded.

Notes

Possibility of read-thru of the pBAD during transcription.

Source

(BBa_B0030), (BBa_B0015), (BBa_R0010), (BBa_I13453), (BBa_J31007), (BBa_J3101)- from registry. (BBa_J31001) - from Davidson College. (BBa_J44000) - made in collaboration with Missouri Western/Davidson.

Components

Access	Instance	Definition
public public public public public public public public public public public	BBa_R0010 BBa_B0030 BBa_J31001 BBa_B0015 BBa_J44000 BBa_I13453 BBa_J44000 BBa_B0030 BBa_J31007 BBa_B0015 BBa_J3101	LacI BBa_B0030 Hin LVA BBa_B0015 hixC BBa_I13453 hixC BBa_B0030 tetA(C)f BBa_B0015 RE

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010 BBa_B0030 BBa_J31001 BBa_B0015 BBa_J44000 BBa_I13453 BBa_J44000 BBa_B0030 BBa_J31007 BBa_B0015 BBa_J3101	1,200 209,223 230,841 850,978 987,1012 1021,1150 1159,1184 1193,1207 1214,2404 2413,2541 2550,2626	LacI BBa_B0030 Hin LVA BBa_B0015 hixC BBa_I13453 hixC BBa_B0030 tetA(C)f BBa_B0015 RE