SynBioHub

Created by: Trevor Y. H. Ho,Baojun Wang

pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry(1-192)-GAEY-e-NpuNcage-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-NpuCcage-CFNS-mCherry(193-236)-H6-Ter

Details

Types	circular DnaRegion
Roles	engineered_plasmid
Sequences	IBMc335_seq (Version 1)

Description

pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-GAEY-e-NpuNcage-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-NpuCcage-CFNS-mCherry*(193-236)-H6-Ter
Expression plasmid for mCherry split at 192/193 inserted with Npu DnaE, caged intein fused with acVHH

Notes

Creator: Trevor Y. H. Ho
Principal Investigator: Baojun Wang
BioSafety Level: Level 1
Backbone: pSB3T5
Origin of replication: p15A
Selection Markers: Tetracycline/ Chloramphenicol

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
mCherry(193-236) ECK120033737 BCD1 tetR promoter acVHH RBS for CDS2 mCherry(1-192) acVHH RBS for CDS 1 PBAD(SapI-) p15A origin of replication RiboJ araC VR RFC10 Prefix (full) PBAD(SapI-) RFC10 Suffix L3S2P55 tetR ECK120033736 NpuN(E52K,E54K,D85K,E89R,E91K) cat promoter H6 NpuC cat (BsmBI-) BBa_B0030 ECK120029600 Caspase3 cut site VF2 (NpuC1-30(K2E,R6E,K11E))x2 Caspase3 cut site NpuN(51-102)	6416,6547 complement(4350,4406) 5641,5725 9,97 5729,6085 5711,5719 2735,3309 3884,4240 5660,5667 2422,2705 7211,8035 5566,5640 complement(1516,2394) complement(6745,6764) 1494,1515 5277,5560 6630,6650 5201,5257 98,1291 6577,6629 3323,3628 complement(5095,5192) 6548,6565 6299,6412 complement(4435,5094) 2714,2728 4252,4341 6272,6283 1371,1390 3680,3862 3644,3655 6101,6256	CDS terminator biological_region promoter CDS ribosome_entry_site CDS CDS ribosome_entry_site promoter origin_of_replication insulator CDS primer_binding_site biological_region promoter biological_region terminator CDS terminator CDS promoter CDS CDS CDS ribosome_entry_site terminator CDS primer_binding_site CDS CDS CDS

Other Properties

genbank#date	18-NOV-2020
genbank#molecule	ds-DNA
synbiohub#ownedBy	user/trevorho
synbiohub#topLevel	IBMc335/1

Member of these Collections

Intein_assisted_Bisection_Mapping

IBMc335

IBMc335 Version 1

Component

Created by: Trevor Y. H. Ho,Baojun Wang

pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry(1-192)-GAEY-e-NpuNcage-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-NpuCcage-CFNS-mCherry(193-236)-H6-Ter

Details

Description

Notes

Sequence Annotations

Other Properties

Member of these Collections

Attachments

Sequence Visualization

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